相关实验视频

Updated: Jun 11, 2026

Combination Radiotherapy in an Orthotopic Mouse Brain Tumor Model

Combination Radiotherapy in an Orthotopic Mouse Brain Tumor Model

Published on: March 6, 2012

在老鼠大脑中开发一个用于马刀放射手术的实验平台

Yueshan Feng^1,2, Jiaxing Yu^1,2, Lixin Xu^1,2

¹Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China.

Radiation research

|May 12, 2025

概括

一个新的3D打印平台可以在小鼠模型中实现精确的Leksell Gamma Knife放射性手术 (GKRS). 计划的临床剂量为40 Gy可能表明小鼠辐射诱导的大脑损伤的门.

科学领域:

神经外科神经外科
辐射瘤学辐射瘤学
临床前研究临床前研究

背景情况:

来自小动物的玛刀放射外科手术后 (GKRS) 样本的有限可用性阻碍了研究.
现有的临床GKRS设备不适合小型动物模型,需要专门的设备.

研究的目的:

开发和验证一种新的3D打印平台,用于在小鼠模型中应用临床GKRS.
建立一个临床前模型,用莱克塞尔马刀研究辐射诱导的脑损伤.

主要方法:

一个3D打印的定位和头部固定装置是为Leksell Gamma Knife设计的.
C57BL/6N小鼠接受了针对特定大脑区域 (CPu,前额基) 的单剂量辐射,预计临床剂量从10-80 Gy.
进行了组织病理学 (H&E, γ-H2AX, CD68, IBA1, NeuN) 和MRI分析以评估辐射效应.

主要成果:

这种新的平台能够在小鼠大脑中准确地输送GKRS,实际剂量比计划高1.5倍.
在计划的临床剂量为50 Gy及以上时,观察到辐射死亡,出血,组织损失和.
显著的DNA损伤 (γ-H2AX) 和神经炎症 (小质/巨细胞激活,神经元死亡) 在计划剂量为40 Gy及以上时发生.

结论:

成功开发了一种用于Leksell Gamma Knife放射性外科手术 (GKRS) 的新型小鼠平台,允许精确的临床前脑辐射.
计划的临床中心剂量40 Gy似乎是诱导成年C57BL / 6N小鼠辐射诱导的大脑损伤的门.

更多相关视频

PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator

PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator

Published on: December 28, 2017

A High-Throughput Image-Guided Stereotactic Neuronavigation and Focused Ultrasound System for Blood-Brain Barrier Opening in Rodents

A High-Throughput Image-Guided Stereotactic Neuronavigation and Focused Ultrasound System for Blood-Brain Barrier Opening in Rodents

Published on: July 16, 2020

相关概念视频

Drug Discovery: Overview

Drug Discovery: Overview

Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...

Structure-Activity Relationships and Drug Design

Structure-Activity Relationships and Drug Design

Drug design is a dynamic field that involves discovering and developing new medications based on specific biological targets. This process heavily relies on structure-activity relationships (SAR) and quantitative structure-activity relationships (QSAR) to guide the design and optimization of efficient drugs.
SAR studies the intricate relationship between a drug's chemical structure and biological activity. It focuses on understanding how modifications to a drug's structure can influence its...