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Updated: May 16, 2025

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Probiotic Studies in Neonatal Mice Using Gavage
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在怀孕维持中产生丁酸盐的细菌:减轻因失生症引起的早产
Azusa Uchida1, Kenji Imai2, Rika Miki3,4
1Departments of Gynecology and Obstetrics, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
Journal of translational medicine
|May 12, 2025
概括
孕产妇的肠道功能障碍症通过降低免疫耐受性,增加了早产风险. 酸盐补充剂,一种肠道细菌的产物,有效地逆转了这种情况,突出了其在饮食干预中预防早产的潜力.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 生殖科学 生殖科学
背景情况:
- 过早分娩 (PTB) 是新生儿并发症的主要原因,其发病率高,原因不明.
- 母亲的肠道微生物群越来越多地参与怀孕维持和免疫调节.
- 失生症可能会破坏免疫耐受性,导致PTB,酸盐可能会恢复调节性T细胞 (Treg) 功能.
研究的目的:
- 调查孕产妇肠道失调在PTB中的因果作用.
- 为了确定酸盐补充剂是否可以预防或减轻PTB.
- 探索肠道微生物群组成与人类PTB之间的关联.
主要方法:
- 一个小鼠模型的PTB被诱导由万科米辛和抗CD3ε抗体,与一些小鼠接受一个富含丁酸盐的饮食.
- 孕期长度,PTB发生率和Treg细胞水平被评估.
- 16S rRNA基因测序分析了来自孕妇的便样本,其中包括患有PTB的孕妇和产期妇女.
主要成果:
- 在小鼠中,dysbiosis导致Treg细胞减少和PTB增加 (43.3%),而酸盐补充剂减少了PTB发病率 (p=0.03) 并恢复了Treg水平 (p<0.001).
- 人类PTB病例显示,酸盐生产细菌 (Lachnospiraceae,Ruminococcaceae) 的丰富性减少.
- 减少酸盐产生细菌与PTB风险独立相关 (p=0.019) 并与妊娠年龄相关 (r=0.59,p<0.001).
结论:
- 孕产妇失生症通过免疫耐受性受损而增加PTB风险,这种机制在体内被丁酸逆转.
- 人类数据证实了生产酸盐的微生物群对维持怀孕的相关性.
- 丁酸盐作为一种饮食干预目标,有望通过恢复免疫平衡来减少PTB.
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