评估野火产生的溶解有机分子的化学反应性:通过肯德里克质量缺陷分析结合氨酸
Hannah M Hamontree1, Patrick G Hatcher1
1Department of Chemistry & Biochemistry, Old Dominion University, 4501 Elkhorn Ave, Norfolk, Virginia 23529, United States of America.
概括
野火产生的溶解有机物 (PyDOM) 可能有毒. 这项研究发现,热性有机物与关键的细胞抗氧化剂谷氨发生反应,这表明生物体中PyDOM毒性的机制.
科学领域:
- 环境化学环境化学
- 毒理学 毒理学 毒理学
- 生物质分析 生物质分析
背景情况:
- 森林火灾会产生由热素衍生的溶解有机物 (PyDOM),由于潜在的毒性和变异性而带来风险.
- 皮多姆可以通过耗尽细胞中的谷氨,一种重要的抗氧化三,诱导动物中的亲氧化反应.
研究的目的:
- 为了研究减少的l-Glutathione和生物质液中的有机分子之间的化学相互作用.
- 用先进的质谱学识别结合机制并量化谷氨和PyDOM组件之间的反应.
主要方法:
- 使用负模式电喷射电离-里埃转换-离子循环子共振质谱学 ((-) ESI-FT-ICR-MS) 来生成分子公式列表.
- 应用了肯德里克质量缺陷 (KMD) 分析,以识别和表征谷与有机分子的结合,在烧焦和未烧焦的生物质液中.
- 在实验室条件下模拟PyDOM的形成和与谷氨的反应.
主要成果:
- 在与谷氨反应后,在烧焦的生物质液中观察到含和硫的分子配方增加了十倍.
- KMD的分析表明,谷氨结合约占新形成的含和硫分子的25%.
- 确定了谷和PyDOM组分之间的添加类型和凝结/消除类型反应.
结论:
- 这项研究阐明了PyDOM毒性的特定化学途径,涉及谷氨酸耗尽.
- 这些发现突出了野火产生的有机化合物的反应分数,这与未来的毒理学评估有关.
- 在KMD分析中,有机物和生物分子之间的复杂相互作用被证明是有效的.
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