干血斑分析用于同时定量抗药物使用液体染色学-并联质谱法
Mariam M Abady1,2,3, Ji-Seon Jeong1,2, Ha-Jeong Kwon1
1Organic Metrology Group, Division of Chemical and Material Metrology, Korea Research Institute of Standards and Science, Daejeon, Republic of Korea.
Rapid communications in mass spectrometry : RCM
|May 13, 2025
概括
干血点 (DBS) 采样为监测抗药物提供了一种实用的,最少侵入性的方法. 这种经过验证的LC-MS/MS方法确保了准确的量化,支持DBS作为治疗药物管理的先进工具.
科学领域:
- 临床化学 临床化学
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 干血点 (DBS) 采样对抗药物 (AED) 的治疗药物监测 (TDM) 具有实际优势.
- 验证DBS的准确性和可靠性对于精确的治疗管理至关重要.
- 在DBS分析中的物理化学挑战需要强大的方法.
研究的目的:
- 为了验证DBS采样用于11种抗药物的定量分析.
- 克服与DBS矩阵相关的分析挑战.
- 建立DBS作为AED治疗药物监测的可靠方法.
主要方法:
- 液体染色学-双重质谱法 (LC-MS/MS) 用于分析.
- 一种基于乙尼的提取方法被优化为DBS样品.
- 验证包括对准确性,精度,选择性,矩阵效应,转移和稳定性的评估.
主要成果:
- 11台AED的提取效率高,精度/精度在6%以内,可以实现.
- 该方法表现出良好的选择性,最小的矩阵效应和微不足道的转移.
- 在室温下,AED在DBS中保持至少30天的稳定性;3毫米的穿孔产生了准确的结果.
结论:
- 使用DBS的优化LC-MS/MS方法对于AED量化是准确,精确和可靠的.
- DBS采样为传统全血监测提供了一种时间和成本高效的替代方案.
- 这支持DBS作为一种有希望的,先进的抗药物治疗药物监测方法.
相关概念视频
Capillary Electrophoresis: Applications
296
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
296
Electrophoresis: Overview
570
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
There...
570
Peptide Identification Using Tandem Mass Spectrometry
6.2K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.2K


