韦塞拉混通过调节炎症路径和肠道微生物群来缓解小鼠实验性结肠炎
Huijing Tang1,2, Qianhua Fan1,2, Yao Lu2
1Department of Epidemiology, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, China.
Frontiers in microbiology
|May 13, 2025
概括
在小鼠模型中,益生菌Weissella confusa Wc1982补充改善了结肠炎症状并改变了肠道微生物群. 这表明W. confusa可能是炎症性肠病 (IBD) 的有前途的治疗策略.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 炎症性肠病 (IBD) 是一种无法治愈的慢性疾病,益生菌正在成为潜在的治疗途径.
- 在临床前模型中,W. confusa表现出已知的抗菌,抗炎和抗氧化特性.
- 在宿主细胞水平上,W. confusa的特定抗炎机制及其对肠道微生物群组成的影响仍然不完全理解.
研究的目的:
- 为了研究韦塞拉混 Wc1982对炎症的治疗作用.
- 分析W. confusa Wc1982对肠道微生物群变化的影响,在硫酸 (DSS) 诱导的大肠炎小鼠模型中.
主要方法:
- 建立了一个DSS诱导的大肠炎小鼠模型,随机分配小鼠到对照,DSS或W. confusa Wc1982治疗组.
- 评估疾病活动指数 (DAI),体重,结肠长度和组织学分数,以评估结肠炎的严重程度.
- 分析了肠道微生物群组成 (16S rRNA测序),结肠基因表达 (RNA-seq) 和细胞因子水平 (ELISA),以确定治疗效应.
主要成果:
- W. confusa Wc1982显著改善了结肠炎,减少了DAI,病理评分和结肠缩短.
- 用W. confusa Wc1982治疗降低了IL-17,IL-6,TNF-α和血清脂多糖的结肠水平.
- W. confusa Wc1982调节了肠道微生物群,增加了alpha多样性和W. confusa和Akkermansia muciniphila的丰富性,同时减少了Enterococcus faecalis和Escherichia coli.
结论:
- 补充Weissella confusa Wc1982显示了缓解大肠炎的显著潜力.
- W. confusa Wc1982对宿主微生物相互作用和大肠炎的炎症途径产生积极影响.
- 这些发现支持W. confusa作为一种有前途的益生菌战略,用于管理炎症性肠病.
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