一个多功能基因工具包,用于工程Wickerhamomyces ciferrii用于四乙烯基植物素生产
Seong-Rae Lee1, Jun Su Kang1, Pyung Cheon Lee1
1Department of Molecular Science and Technology and Advanced College of Bio-convergence Engineering, Ajou University, Suwon, Republic of Korea.
Frontiers in bioengineering and biotechnology
|May 13, 2025
概括
研究人员为Wickerhamomyces ciferrii开发了一套遗传工具,Wickerhamomyces ciferrii是一种具有产业潜力的酵母,可以生产四乙基植物素 (TAPS). 这个系统可以实现应变工程,并通过代谢途径优化来增强TAPS的生产.
科学领域:
- 工业生物技术 工业生物技术
- 酵母遗传学 酵母遗传学
- 合成生物学 合成生物学
背景情况:
- Wickerhamomyces ciferrii是一种非传统的酵母,具有显著的工业潜力,特别是在生产四乙烯基植物素 (TAPS) 方面.
- 它的工业应用目前由于缺乏全面的基因工程工具箱而受到限制.
研究的目的:
- 开发Wickerhamomyces ciferrii的模块化遗传系统,以促进菌株工程和代谢途径优化.
- 建立一个强大的基因平台,以提高TAPS等有价值化合物的生产.
主要方法:
- 用可选择标记物,复制原点和光报告器构建和评估附带性等离子体.
- 对抗生素耐药性标志物和W. ciferrii的复制起源进行系统查.
- 在内源性促进体下对光蛋白表达的分析和子优化效应.
- 通过过度表达乙-CoA碳酸酶 (ACC1) 来增加TAPS产量的功能验证.
主要成果:
- 诺尔西奥素,遗传素和热素被确定为有效的可选择标记物; 素B是无效的.
- 2μ和CEN6/ARS4复制起源支持稳定的插曲性维持.
- 对于异质基因表达来说,子优化至关重要,正如光蛋白的可变转录水平所示.
- 过度表达ACC1导致TAPS产量增加了2.4倍,证明了工具包的有效性.
结论:
- 已经建立了Wickerhamomyces ciferrii的多功能和模块化遗传系统.
- 这个工具包为未来的合成生物学和代谢工程应用在这种酵母物种中提供了坚实的基础.
- 开发的系统显著提高了TAPS工业生产的潜力.
相关概念视频
00:49What is Genetic Engineering?
72.8K
Overview
72.8K
02:53Conservative Site-specific Recombination and Phase Variation
5.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
5.9K
01:20The Central Dogma
19.4K
The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
19.4K
01:16In-vitro Mutagenesis
13.6K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
13.6K
02:50Transgenic Plants
7.1K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.1K