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韦塞拉混 XG-3 EPS:通过与Candida shehatae共同培养和部分表征来加强生产

Shuhan Yang¹, Shouqi Zhao¹, Yingying Ning¹

¹Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin, China.

Preparative biochemistry & biotechnology

|May 13, 2025

概括

这项研究优化了Weissella confusa和Candida shehatae的共同培养,以促进外聚糖 (EPS) 产量. 增强的EPS表现出卓越的功能性质,包括抗氧化活性和益生菌支持,用于食品工业应用.

科学领域:

微生物学微生物学
生物技术是生物技术.
食品科学食品科学食品科学

背景情况:

共同培养方法可以增强外聚糖 (EPS) 产量.
之前的研究没有报告EPS增强在Weissella confusa和Candida shehatae的共同培养中.

研究的目的:

为 W. confusa 和 C. shehatae 开发一个高效的共同培养系统.
通过响应表面方法来优化EPS产量和性能.
评估共同培养的EPS的功能特征.

主要方法:

在W.confusa XG-3和C.shehatae的共同培养中,其比例为1:1.
响应表面的方法来优化糖糖,酵母提取物和的度.
分析EPS产量,水溶性,水容量,抗氧化活性,益生菌增殖效应和热稳定性.

主要成果:

最初的共同培养产生了56.82g/L的EPS,比单一培养增加了1.63倍.
优化条件 (105.97 g/L 糖,5.81 g/L 酵母提取物,6.24 g/L ) 提高了产量,达到115.66 g/L (2.04 倍的改善).
同种植的EPS表现出高水溶性 (60.45%),保持水容量 (614.74%),显著的抗氧化活性,优越的益生菌增殖支持,以及高达292.5°C的热稳定性.

结论:

建立了一个新的和可扩展的共同文化战略,用于生产多功能EPS.
优化的EPS具有适用于各种食品工业应用的理想特性.
这种方法为提高EPS生产和功能提供了一个有希望的方法.

关键词:

共同文化是一种共文化.威塞拉混 (Weissella confusa) 是一种令人困惑的植物.标志性特征的描述.异型多糖糖化物外型多糖化物响应表面方法的方法.

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