相关实验视频

Updated: May 14, 2025

Measurement of Pulse Propagation Velocity, Distensibility and Strain in an Abdominal Aortic Aneurysm Mouse Model

Measurement of Pulse Propagation Velocity, Distensibility and Strain in an Abdominal Aortic Aneurysm Mouse Model

Published on: February 23, 2020

环-LRP6通过miR-29a-3p/HIF-1α轴抑制AAA的发展和进展

Fang Wang¹, Zhijian Sun², Wenke Yan²

¹Department of Pathology, Qilu Hospital of Shandong University, Dezhou Hospital, Dezhou, Shandong Province, China.

Current pharmaceutical biotechnology

|May 13, 2025

概括

循环RNA LRP6 (circLRP6) 通过调节miR-29a-3p/HIF-1α通路来抑制腹腔大动脉动脉瘤 (AAA) 的发展. 过度表达circLRP6显示了AAA治疗的治疗潜力.

科学领域:

分子生物学分子生物学
心血管研究研究心血管研究
生物化学生物化学

背景情况:

腹腔大动脉动脉瘤 (AAA) 的发病因子仍然不明,这限制了有效的治疗策略.
循环RNAs (circRNAs) 正在成为各种疾病的关键调节剂和潜在的诊断生物标志物.
关于AAA背后的分子机制的研究有限,突出了对新见解的需求.

研究的目的:

在腹腔大动脉动脉瘤 (AAA) 的背景下研究circLRP6的分子机制.
探索circLRP6作为AAA的潜在治疗点.
为优化AAA的临床治疗提供理论基础.

主要方法:

为AAA建立动物和细胞模型.
使用in situ杂交和qRT-PCR验证circLRP6的表达.
通过CCK-8,BrdU和流细胞测量评估细胞活力,细胞循环和细胞亡.
证实circLRP6与miR-29a-3p和HIF-1α的相互作用,使用 luciferase 记者基因和 RIP 试验.
通过免疫光和西部斑分析检测HIF-1α和caspase 3表达.

主要成果:

在AAA模型中观察到减少circLRP6表达.
过度表达circLRP6显著抑制AngII诱导的人类大动脉光滑肌细胞 (hAoSMC) 的活力.
生物信息学和实验验证证证了HaoSMCs中circLRP6和miR-29a-3p之间的负面调控相互作用.
发现circLRP6与HIF-1α相互作用,共同抑制miR-29a-3p/HIF-1α轴以实现有效的AAA治疗.

结论:

在抑制AAA进展方面,CircLRP6起着至关重要的作用.
循环LRP6对AAA的抑制作用通过抑制miR-29a-3p/HIF-1α通路进行介导.
这些发现为AAA治疗策略的临床优化提供了理论基础.

关键词:

在CircLRP6中使用.循环RNA 是一个循环RNA.这就是HIF-1α.腹部大动脉动脉瘤腹部大动脉动脉瘤在 miR-29a-3p 中.

更多相关视频

Porcine Model of Infrarenal Abdominal Aortic Aneurysm

Porcine Model of Infrarenal Abdominal Aortic Aneurysm

Published on: November 21, 2019

Ultrasound Imaging of the Thoracic and Abdominal Aorta in Mice to Determine Aneurysm Dimensions

Ultrasound Imaging of the Thoracic and Abdominal Aorta in Mice to Determine Aneurysm Dimensions

Published on: March 8, 2019

相关概念视频

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...