对脊椎动物宿主特异性Lactobacillus johnsonii在胃肠道中的适应性进行基因组和转录组洞察
Keerthikka Ravi1, Nicole R Falkowski2,3, Gary B Huffnagle1,2,3
1Department of Molecular, Cellular & Developmental Biology, University of Michigan, Ann Arbor, Michigan, USA.
mSphere
|May 13, 2025
概括
牛乳杆菌 johnsonii 菌株显示与宿主相关的遗传差异,动物相关的基因在小鼠殖民期间活跃和调节. 这突显了细菌适应特定环境的特点.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 宿主-微生物相互作用
背景情况:
- 牛乳杆菌 (Lactobacillus johnsonii) 是一种具有医学意义的益生菌,需要了解其宿主适应.
- 细菌利基适应涉及基因组变化,包括基因增加/损失和尺寸改变.
研究的目的:
- 探索来自各种脊椎动物胃肠道的Lactobacillus johnsonii菌株宿主特异性的遗传基础.
- 在各种生长条件下,研究动物特异性基因在Lactobacillus johnsonii MR1中的表达.
主要方法:
- 来自动物,禽类,猪类和人类宿主的Lactobacillus johnsonii菌株的比较基因组分析.
- 在体外 (无氧,37°C,指数/静止阶段) 和体内 (老鼠) 生长过程中对Lactobacillus johnsonii MR1的转录分析.
主要成果:
- 观察到显著的基因组异质性,与动物或鸟类起源相关的基因组聚类.
- 与鸟类分离物相比,动物分离物显示了表面蛋白质,辅助分泌途径基因和氨酸脱酶基因的丰富.
- 40个已识别的动物相关基因在Lactobacillus johnsonii MR1中转录活跃,在特定的生长阶段和体内殖民期间有差异性调节.
结论:
- 牛乳杆菌 johnsonii 菌株表现出与其宿主物种相关的基因组内容变异.
- 不同的基因转录表明适应特定的宿主环境和营养素的可用性.
- 已识别的基因在营养适应和宿主微生物相互作用中发挥潜在作用,特别是在动物宿主中.
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