功能性灭绝的北方白犀牛 (Ceratotherium simum cottoni) 的基因组图
Gaojianyong Wang1,2,3, Marisa L Korody4, Björn Brändl2,3
1Department of Genome Regulation, Max Planck Institute for Molecular Genetics, Berlin 14195, Germany.
概括
北方白犀牛的基因组被测序以帮助保护. 这种参考基因组可以评估诱导的多能干细胞完整性,用于潜在的辅助生殖和物种恢复.
科学领域:
- 保护基因组学 保护基因组学
- 干细胞生物学 干细胞生物学
- 生殖技术 生殖技术
背景情况:
- 北方白犀牛 (NWR) 处于极度危状态,面临功能性灭绝.
- 诱导多能干细胞 (iPSCs) 提供了通过体外生殖细胞生成 (in vitro gametogenesis) 来保护NWR的潜力.
- NWR iPSCs的基因组完整性对于成功的辅助生殖技术至关重要.
研究的目的:
- 为NWR产生高质量的染色体水平参考基因组.
- 建立一种方法来评估NWR iPSCs的基因组完整性.
- 为了支持NWR的保护工作.
主要方法:
- 使用互补测序和映射生成染色体级NWR参考基因组.
- 与马的基因组进行比较的FISH映射.
- 与南方白犀牛 (SWR) 基因组对齐.
- 与纤维细胞相比,对NWR iPSCs中的拷贝数变化的分析.
主要成果:
- 建立了一个全面的NWR参考基因组,包括自体,性染色体和线粒体DNA.
- 在NWR和马基因组之间观察到高水平的染色体保护.
- NWR和SWR基因组在染色体水平上显示出显著的相似性.
- 在NWR iPSC线路中发现了副本数变异,可能是由于体外扩张.
结论:
- NWR参考基因组为评估NWR iPSC基因组完整性提供了一个关键工具.
- 这种评估对于指导用于辅助生殖的iPSC差异化至关重要.
- 该基因组序列将有助于非凡的保护策略,包括克隆和iPSC衍生性雌同体应用.
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