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相关概念视频

Coat Assembly and GTPases01:33

Coat Assembly and GTPases

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Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
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GTPases and their Regulation02:14

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Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒  small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
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Rab Cascades01:25

Rab Cascades

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Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
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Small GTPases - Ras and Rho01:24

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Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
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Vesicular Tubular Clusters01:45

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After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
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Rab Proteins01:14

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Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
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相关实验视频

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Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
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TORC1通过Rag GTPase tether Tco8989自主控制其空间分区.

Raffaele Nicastro1, Marie-Pierre Péli-Gulli1, Marco Caligaris1

  • 1Department of Biology, University of Fribourg, Fribourg, Switzerland.

Cell reports
|May 13, 2025
PubMed
概括

酵母利用Tco89将TORC1与Rag GTPases结合起来,调节Sch9激酶的活性. 通过酸化,TORC1稳定了Tco89,防止其降解,并维持TORC1的营养感应本地化.

关键词:
CP: 细胞生物学 细胞生物学科普:分子生物学 分子生物学拉格 GTPases 的使用方法在TORC1中,在Tco8989中使用.氨基酸信号传递方式增长控制 增长控制 增长控制拉帕米辛复合物的目标1 1

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Last Updated: May 15, 2025

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
10:27

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科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 拉巴胺素复合体1 (TORC1) 的标是细胞生长和恒温的关键调节者.
  • TORC1集成营养信号,特别是氨基酸的可用性,在内分泌系统.
  • 在哺乳动物中,Rag GTPases通过猛禽子单元将TORC1招募到溶解体中,这是激活必不可少的过程.

研究的目的:

  • 研究酵母TORC1被招募到活性Rag GTPases的机制.
  • 在没有哺乳动物猛龙爪域的情况下,识别负责将TORC1与Rag GTPases结合的酵母骨科.
  • 为了阐明TORC1如何调节其效应酶Sch9以响应氨基酸可用性.

主要方法:

  • 在酵母 (Saccharomyces cerevisiae) 中进行基因分析.
  • 酸化部位映射和蛋白质分解研究.
  • 使用显微镜进行亚细胞局部化测试.
  • 生物化学测试以评估酶活性.

主要成果:

  • 酵母使用真菌特异性蛋白Tco89将TORC1绑定到活性Rag GTPases,与哺乳动物猛龙爪域不同.
  • Tco89充当支架,使TORC1能够酸化和稳定自己,从而校准Sch9激酶活性.
  • TORC1的失活导致Tco89蛋白解,导致TORC1从真空细胞再分配到信号内分泌体,并从Sch9.9空间分离.

结论:

  • 酵母TORC1利用Tco89进行溶酶体招募,显示出一种保留但与哺乳动物不同的机制.
  • TORC1动态控制Tco89的稳定性及其自身的亚细胞局部化,以适应营养的波动.
  • 这种空间调节允许通过在营养物质稀缺时将TORC1与其效应器空间分开来节约能量.