微生物生物合成罕见的大麻素
Chunsheng Yan1, Ikechukwu C Okorafor1,2, Colin W Johnson3
1Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA 90095, USA.
Journal of industrial microbiology & biotechnology
|May 13, 2025
概括
研究人员设计了Saccharomyces cerevisiae以从简单的糖中产生罕见的大麻素,如∆9-四化甘生物醇酸和∆9-四化甘醇. 这种微生物平台提供了一种可持续的方法来获取有价值的大麻化合物.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 微生物工程 微生物工程
背景情况:
- 大麻植物产生丰富的大麻素,如∆9-四大麻 (∆9-THC) 和大麻二醇.
- 具有独特基链的罕见的∆9-THC类似物具有强大的生物活性,但很难分离或合成.
- 微生物生物合成为生产这些罕见的大麻素提供了一个有希望的替代方案.
研究的目的:
- 开发一种Saccharomyces cerevisiae宿主,用于罕见大麻素的微生物生物合成.
- 为了设计酵母,有效地生产特定的罕见大麻素酸.
- 探索这个平台在产生新型大麻素方面的潜力.
主要方法:
- 经过工程设计的Saccharomyces cerevisiae,可以积累兰酸铁酸盐.
- 过度表达了一种用于2,4-二基-6-基酸合成的真菌通路.
- 引入了UbiA-prenyltransferase和∆9-tetrahydrocannabinolic 酸合成酶基因.
主要成果:
- 成功生物合成了两种罕见的大麻素酸:C1替代的∆9-四氨基可纳比奥里克酸 (∼16毫克/升) 和C7替代的∆9-四氨基可纳比酸 (∼5毫克/升).
- 热去碳化∆9-四化甘二醇酸以产生∆9-四化甘二醇.
- 证明了使用工程酵母从简单糖中生产罕见的大麻素的可行性.
结论:
- 改造的Saccharomyces cerevisiae作为一种有效的宿主,可以生产罕见的大麻素.
- 微生物平台有潜力通过利用真菌基因集群生产多种罕见和新型大麻素.
- 这种方法提供了一种可持续和可扩展的方法来获取有价值的大麻素化合物.
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