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用 CrUGT74AN3 和 BlCGTase 催化的大麻素甘化方法

Christina Schmidt¹, Astrid Maria Imann^1,2, Nikolay Vasilev¹

¹Technical Biochemistry Laboratory, Department of Biochemical and Chemical Engineering, TU Dortmund University, Dortmund, Germany.

Biotechnology journal

|May 13, 2025

概括

这项研究通过使用工程酵母系统进行糖化,提高可溶性和稳定性来增强大麻素 (CBD) 制药潜力. 该系统有效地产生CBD糖化物,对其他大麻素也显示出希望.

科学领域:

生物技术和生物化学
酶学是一种酶学.
代谢工程是代谢工程.

背景情况:

植物性大麻素 (phytocannabinoids),就像大麻素 (Cannabis sativa) 中的植物性大麻素 (phytocannabinoids) 一样,具有制药潜力,但由于脂性,其生物利用性较低.
糖化增强了脂性分子的水溶性和稳定性.
有效的酶基糖化需要高度活跃的酶和强大的生产系统,很少有已知的甘氨酸转移酶具有大麻素活性.

研究的目的:

为了探索各种大麻素的UDP-glycosyltransferase CrUGT74AN3的基质范围.
建立一个工程酵母系统,以有效地实现大麻素生物转化和糖化.
通过解决诸如葡萄糖酶活性和UDP-葡萄糖供应之类的局限性来优化糖化产量.

主要方法:

选乱交的CRUGT74AN3酶对一系列大麻素的活性.
工程Saccharomyces cerevisiae用于增强大麻 (CBD) 的生物转化.
调查和缓解酵母糖化系统的局限性,包括共同表达策略.

主要成果:

CrUGT74AN3对大麻素表现出广泛的活性,使用大麻 (CBD) 的效率最高.
工程酵母有效地产生了CBD-O-糖化物,与循环德氨酸糖转移酶的共同表达产生了多达6个葡萄糖单位的CBD-糖化物.
该系统的适用性已被证实用于其他大麻素,包括cannabigerol和cannabinol.

结论:

工程酵母系统为生产糖化大麻素提供了一个高效的平台,增强它们的制药特性.
CrUGT74AN3 是一种用于大麻素糖化的一种多功能酶,适用于各种化合物.
这种生物转化方法提供了一种可扩展的方法来提高大麻素的溶解性和稳定性.

关键词:

生物转化过程中的生物转化.大麻素是一种大麻素.葡萄糖酶化是什么? 葡萄糖酶化是什么?代谢工程是代谢工程.酵母酵母是一种酵母.

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