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在GV-MI过渡期间对人类卵细胞进行单细胞蛋白质组学分析

Zeling Zhang¹, Lei Luo¹, Shiwei Fan¹

¹Division of Life Sciences and Medicine, Department of Obstetrics and Gynecology, Center for Reproductive and Genetics, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, China.

Human reproduction (Oxford, England)

|May 13, 2025

概括

研究人员在人类卵细胞成熟过程中从生殖囊泡 (GV) 到元相I (MI) 阶段确定了149种不同表达的蛋白质. 这项研究增强了对卵细胞发育的理解,并可能改善体外成熟技术.

科学领域:

人类卵细胞发育的蛋白质组学和分子生物学.
介质变化的细胞和分子机制.
生殖生物学和辅助生殖技术.

背景情况:

人类卵细胞的成熟涉及存储早期胚胎发育的母性因素.
在实验室成熟的生殖囊泡 (GV) 卵细胞的芽细胞发育率低于元相I (MI) 卵细胞.
了解GV-MI过渡期间的蛋白质变化对于改善卵细胞利用是至关重要的.

研究的目的:

为了识别在人类卵细胞从生殖囊泡 (GV) 过渡到元相I (MI) 阶段时差异表达的蛋白质.
了解这些蛋白质在卵细胞成熟中的功能作用.
探索改善体外成熟培养基的潜在应用.

主要方法:

16个人类卵细胞 (8GV,8MI) 的液体染色学-并联质谱学 (LC-MS/MS) 分析.
使用生物信息学工具对差异性蛋白质进行选和功能性注释.
免疫光 (IF) 验证差异性蛋白质表达和RNA干扰 (siRNA),以评估蛋白质功能.

主要成果:

鉴定了2369种蛋白质,其中149种具有显著的差异性表达 (MI卵细胞中79种上调,70种下调).
不同表达的蛋白质主要参与细胞代谢,生物调节和信号转导通路.
发现的关键蛋白包括ACTR2,NDUFS1,TUBGCP3和HSPB1,涉及线粒体功能和细胞分裂;TUBGCP3的表达被siRNA降低.

结论:

在人类卵细胞GV到MI过渡期间确定了关键的差异表达蛋白质.
这些蛋白在卵细胞成熟中的功能意义需要进一步阐明.
生成条件淘汰赛小鼠,以研究选定蛋白质在卵细胞成熟中的作用.

关键词:

辅助生殖技术是一种辅助生殖技术.这是生殖囊泡的生殖囊泡.第一个元相I阶段.卵细胞成熟的过程单细胞蛋白组学单细胞蛋白组学

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