用拉曼光谱和解释AI评估血清稳定性的评估
Verónica Mieites1, María Gabriela Fernández-Manteca1, Inés Santiuste Torcida2
1University of Cantabria, Photonics Engineering Group, Plaza de la Ciencia S/N, Santander, 39005, Cantabria, Spain; Valdecilla Health Research Institute (IDIVAL), Calle Cardenal Herrera Oria S/N, Santander, 39011, Cantabria, Spain.
概括
拉曼光谱可以评估血清降解. 自动光背景显示为监测血清储存质量的生物标志物具有前景,即使样本变化.
科学领域:
- 生物医学光谱学 生物医学光谱学
- 分析化学 分析化学
- 生物标志物发现发现
背景情况:
- 血清样本的完整性对于准确的诊断至关重要.
- 存储延迟可能会影响血清分析物的稳定性.
- 需要快速评估血清质量的方法.
研究的目的:
- 评估传统的拉曼光谱检测由于储存而导致的血清降解.
- 评估光谱分析管道对血清质量控制的有用性.
- 探索自发光作为血清储存生物标记物的潜力.
主要方法:
- 血清样本用拉曼光谱在室温下4天内进行分析.
- 用规范化,异常值检测,基线校正和主要组件分析 (PCA) 处理的光谱数据.
- 在不同的数据集上训练和评估分类模型 (KNN,RF,SVM).
主要成果:
- 由于分析物的降解,观察到有限的光谱变化;样本间和样本内变异性占主导地位.
- 分类模型在保留光谱基线的数据集上表现最好.
- 删除基线校正导致不确定的结果.
结论:
- 传统的拉曼光谱显示对血清中微妙的分析物降解的敏感性有限.
- 拉曼光谱中的自发光背景是监测血清储存质量的有希望的指标.
- 强大的光谱分析管道对于可靠的血清样本评估至关重要.
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