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Updated: May 15, 2025

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在Pseudocaranx dentex骨肌的转录组分析和替代拼接分析:洞察慢和快肌肉专业化

Huan Wang¹, Ang Li¹, Changting An¹

¹State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.

International journal of biological macromolecules

|May 13, 2025

概括

这项研究揭示了远距离肌肉中广泛的转录多样性和替代拼接 (AS),揭示了通过后转录调节驱动肌肉类型专业化的关键基因和机制.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
比较生理学比较生理学

背景情况:

替代拼接 (AS) 对于哺乳动物的肌肉类型专业化至关重要,但在远距离动物中没有得到充分的研究.
了解teleost肌肉中的AS对于破译肌肉功能和演变至关重要.

研究的目的:

为了全面地描述转录组和AS景观的缓慢抽肌肉 (SM) 和快速抽肌肉 (FM) 在迁徙的teleost,Pseudocaranx dentex.
鉴定有助于肌肉类型专业化的差异表达转录 (DET) 和差异AS基因 (DASG).
阐明转录后调节在远肌肉适应中的作用.

主要方法:

结合Iso-Seq和RNA-Seq技术用于高通量转录组和AS分析.
在SM和FM之间对转录和拼接事件的差异表达分析.
差异性AS基因 (DASGs) 的识别和功能注释.

主要成果:

在SM和FM中发现了广泛的转录和异形多样性,有数千个全长的转录和异形.
检测到许多差异表达的转录 (DET) 和替代拼接 (AS) 事件,主要是跳过的外子 (SE) 和内子保留 (IR).
发现了325个DASG参与代谢过程,器官组织和肉髓组合,包括tnni2,capzb,neb和pdlim5.

结论:

这项研究提供了第一个全面的转录组复杂性和teleost骨肌肉的拼接变体的观点.
替代拼接显著促进了缓慢缩和快速缩肌肉之间的功能和分子分歧.
通过AS进行的转录后调节在远距离肌肉类型的专业化中起着至关重要的作用.

关键词:

另一个替代拼接方法.快速抽的肌肉 (FM) 是一种肌肉.这就是Iso-Seq.肌肉的专业化肌肉的专业化伪卡朗克斯 (Pseudocaranx dentex) 是一个虚假的字体.缓慢抽肌肉 (SM) 是一种肌肉.

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