在葡萄酒发酵中的不同糖度下对Saccharomyces cerevisiae中更高的酒精生产的核心调节基因的转录基因鉴定
Lu Chen1, Xiaona Ren1, Yanan Wang1
1College of Enology, Northwest A&F University, Yangling 712100, China.
Foods (Basel, Switzerland)
|May 14, 2025
概括
葡萄中的高糖含量显著增加了葡萄酒中较高的酒精产量. 这就是GRE3基因.
科学领域:
- 葡萄酒学 葡萄酒学 葡萄酒学
- 葡萄酒化学 葡萄酒化学
- 发酵科学 发酵科学
背景情况:
- 酒精含量较高的酒精是葡萄酒调味的主要化合物.
- 葡萄糖含量增加会影响葡萄酒的质量.
- 了解糖对较高酒精度的影响至关重要.
研究的目的:
- 调查高糖对较高酒精产量的影响.
- 阐明底层的分子机制.
- 确定参与该过程的关键基因.
主要方法:
- 葡萄酒发酵,使用不同度的糖 (240 g/L, 280 g/L).
- 转录组测序用于基因表达分析.
- 用分子方法进行基因淘汰实验 (GRE3).
主要成果:
- 高糖度 (240g/L,280g/L) 增加了17%和24%的较高的酒精.
- 转录组分析发现GRE3是影响酒精含量更高的关键基因.
- 在240g/L的糖分下,GRE3淘汰将较高的酒精产量降低了17.76%.
结论:
- 葡萄中的高糖含量对葡萄酒中较高的酒精产量产生积极影响.
- 在Saccharomyces cerevisiae中,GRE3基因在调节高酒精合成方面发挥着重要作用.
- 这项研究揭示了新的机制,将葡萄糖水平与葡萄酒香味化合物联系起来.
更多相关视频
07:28An Inexpensive, Scalable Behavioral Assay for Measuring Ethanol Sedation Sensitivity and Rapid Tolerance in Drosophila
Published on: April 15, 2015
10.2K
06:53In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast
Published on: February 21, 2025
271
相关概念视频
Cell Specific Gene Expression
13.3K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.3K
Transcription Attenuation in Prokaryotes
15.0K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.0K
Master Transcription Regulators
6.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.8K
Prokaryotic Transcriptional Activators and Repressors
20.3K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
20.3K
Regulation of Expression at Multiple Steps
849
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
849
Regulation of Expression Occurs at Multiple Steps
22.0K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.0K
