基异硫酸盐和抗菌素对黄金葡萄球菌的协同抗菌机制通过转录基因分析揭示,并在其牛肉中的应用
Jianan Liu1,2, Jinle Ma1,2, Yingrui Wang1
1School of Food Science and Technology, Dalian Polytechnic University, No. 1, Qinggongyuan, Ganjingzi District, Dalian 116034, China.
Foods (Basel, Switzerland)
|May 14, 2025
概括
异硫酸 (BITC) 和复星醇 (RES) 具有对抗黄菌的协同抗菌作用. 这种组合抑制了生长,生物膜的形成,并破坏了细胞膜,提供了一个有前途的抗菌策略.
科学领域:
- 微生物学和分子生物学
- 抗微生物耐药性 抗微生物耐药性
- 食品安全 食品安全
背景情况:
- 黄金葡萄球菌 (S. aureus) 由于抗生素耐药性增加而构成重大威胁.
- 自然化合物,如二酸 (BITC) 和复星 (RES) 显示个人抗菌潜力.
- 了解协同作用机制对于开发新型抗S. aureus策略至关重要.
研究的目的:
- 阐明BITC和RES在转录水平上对S. aureus的协同抗菌机制.
- 评估S. aureus对生长,生物膜形成和细胞膜完整性的综合影响.
- 在食品矩阵中评估BITC-RES组合的抗菌疗效.
主要方法:
- 转录组分析 (RNA测序) 用于识别用BITC,RES和它们的组合 (BITC_RES) 治疗的金黄色菌中的差异表达基因 (DEG).
- 评估细菌生长,生物膜抑制和细胞膜破坏试验.
- 在不同温度 (4°C和25°C) 的新鲜瘦牛肉中评估抗菌活性.
主要成果:
- 与单个治疗相比,BITC_RES组合显著降低了S. aureus的生长,抑制了生物膜的形成,并增加了细胞膜破坏.
- 转录组分析显示,BITC_RES组的DEGs大幅增加,这表明对细菌基因表达有广泛的影响.
- 关键的毒性相关基因,包括血清蛋白酶,SAE调控系统基因,agrC,囊蛋白酶和血解素毒素基因,被BITC_RES组合显著下调.
结论:
- BITC和RES表现出对S. aureus的协同抗菌作用,通过降低关键毒性和调节基因的调节来调节.
- 这种组合有效地抑制金黄色细菌的生长和毒性因素,表明其作为一种新型抗菌剂的潜力.
- 在食品模型中证实了协同作用的抗微生物活性,强调了其在食品保存和安全方面的适用性.
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