轮胎微塑料对有氧颗粒性泥微生物活性的影响
Weronika Irena Mądzielewska1, Piotr Jachimowicz2, Job Oliver Otieno1
1Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Słoneczna 45G, 10-709 Olsztyn, Poland.
International journal of molecular sciences
|May 14, 2025
概括
废水中的轮胎磨损颗粒 (TWPs) 增加了氨的去除,但抑制了有氧颗粒污泥中的关键脱基因. 需要进一步的研究来减轻TWPs.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 环境工程 环境工程
背景情况:
- 轮胎磨损颗粒 (TWPs) 是来自车辆轮胎的新兴微塑料污染物.
- 污水处理厂 (WWTP) 是TWPs进入水环境的重要途径.
- 对于TWPs对生物废水处理过程,特别是代谢的影响,仍未得到充分研究.
研究的目的:
- 研究TWP微塑料对有氧颗粒污泥中代谢基因转录的影响.
- 量化不同TWP度对生物反应器中关键微生物过程的影响.
- 评估TWPs对氨氧化和脱的影响.
主要方法:
- 实验室级测序有氧颗粒污泥反应堆的运行周期为8小时.
- 在50至500毫克/升的度下,TWP微塑料被引入到城市废水中.
- 使用定量PCR分析了16SrRNA,amoA,nirK和nosZ的基因转录水平.
- 监测了氨的去除速度和氧化产品.
主要成果:
- 暴露于TWP导致氨去除率增加和氧化形式度更高.
- 对于氨氧化至关重要的amoA基因的转录在TWP存在时增加了高达160%.
- 脱基因转录,特别是nirK和nosZ受到了负面影响,在最高的TWP剂量下,酸盐减少酶降低了33%,氧化减少酶降低了58%.
结论:
- 在有氧颗粒污泥中,TWPs显著改变微生物基因表达,促进氨氧化细菌,同时抑制脱.
- 这些发现突出了由于TWP污染导致的WWTP中循环的潜在破坏.
- 对TWP对微生物群落影响机制的进一步调查对于有效的废水处理策略至关重要.
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