相关实验视频

Updated: May 17, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

微RNA-29c-3p和-126a有助于降低衰老内皮原生细胞的血管生成潜力

Derek M Dykxhoorn¹, Andrea Da Fonseca Ferreira², Karenn Gomez²

¹John T. Macdonald Foundation Department of Human Genetics and, the John P. Hussman Institute for Human Genomics, University of Miami Miller School of Medicine, Miami, FL 33136, USA.

International journal of molecular sciences

|May 14, 2025

概括

衰老会通过特定的微RNA (miRNA) 途径损害内皮原生细胞 (EPC) 功能和血管修复. 这项研究确定了一个关键的miRNA网络,影响血管内皮生长因子信号传输,对心血管健康至关重要.

科学领域:

心血管生物学心血管生物学
衰老研究研究衰老研究
分子医学是分子医学.

背景情况:

内皮原生细胞 (EPC) 对于血管修复和健康至关重要.
老龄化会减少EPC的数量和功能,增加心血管疾病 (CVD) 的风险.
微RNAs (miRNAs) 越来越多地被认为是EPC功能的关键调节者.

研究的目的:

通过miRNA-mRNA通路调查衰老如何影响EPC健康和功能.
为了确定与年龄相关的EPC功能障碍相关的特定miRNA及其目标.
阐明这些途径在血管修复和心血管疾病中的作用.

主要方法:

从年轻和老年小鼠的EPC中进行微RNA和mRNA分析.
一个涉及miR-29c-3p和miR-126a的监管网络的分析.
功能测定EPC (自我更新,迁移,管形成) 和体内心脏修复模型.

主要成果:

确定了一个与衰老相关的网络,涉及miR-29c-3p和miR-126a.
这些miRNAs通过向Klf2和Spred1.1来损害血管内皮生长因子 (VEGF) 信号传输.
这种途径的调节显著影响了EPC功能和心脏修复.

结论:

该miR-29c-3p-Klf2-miR-126a-Spred1-VEGF轴对EPC功能至关重要.
这一途径调解了EPC驱动的血管修复中与年龄相关的缺陷.
了解这一轴为老年人群中心血管疾病提供了潜在的治疗点.

关键词:

老化的老化衰老的老化血管新生是因为血管新生.内皮前代细胞是内皮前代细胞.

更多相关视频

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery

Published on: February 14, 2017

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

相关概念视频

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...