抗氧化和抗炎活动的Astilboides表表 (Hemsl.) 英语:英格兰. 根源提取物 根源提取物
Nam Ho Yoo1, Young Sun Baek1, Hee Kyu Kim2
1Department of Bio-Resource Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea.
Molecules (Basel, Switzerland)
|May 14, 2025
概括
阿斯蒂尔博伊德 (Astiboides tabularis) 根的乙烯酸盐部分表现出强大的抗氧化和抗炎作用. 它有效地清除激素并抑制炎症途径,这表明它有治疗炎症疾病的潜力.
科学领域:
- 药理学和植物化学
- 自然产品化学 自然产品化学
背景情况:
- 炎症性疾病对全球健康构成重大负担.
- 自然产品是富含抗氧化和抗炎性质的治疗化合物的丰富来源.
- 传统上使用的是Astilboides tabularis根,但其生物活性成分需要科学验证.
研究的目的:
- 调查来自Astiboides tabularis根的乙烯酸乙酸 (EtOAc) 分数的抗氧化和抗炎潜力.
- 确定负责观察到活动的关键生物活性化合物.
主要方法:
- 使用70%的乙醇提取Astilboides tabularis根,然后进行乙烯酸乙烯分离.
- 在体外抗氧化剂测定:DPPH和ABTS激素清除活性,总含量.
- 在实验室使用RAW264.7的抗炎试验中,用脂多糖 (LPS) 刺激的巨细胞,包括氧化 (NO) 生产和炎症媒介表达 (iNOS,COX-2,TNF-α,IL-1β,IFN-β) 分析.
- 使用Western blot和RT-PCR评估NF-κB和MAPK信号通路激活的机制研究.
- 使用LC-QTOF-MS和LC-MS/MS进行化合物识别.
主要成果:
- 该EtOAc分量显示出显著的DPPH (IC50: 11.38μg/mL) 和ABTS (IC50: 7.46μg/mL) 激素清除活性,总含量高 (407.02毫克GAE/g).
- 该分子在RAW264.7细胞中表现出度依赖的抗氧化应激保护作用.
- 它显著抑制了LPS刺激细胞中的NO生产和关键炎症媒介体 (iNOS,COX-2,TNF-α,IL-1β,IFN-β) 的表达.
- 抑制炎症媒介表达与抑制NF-κB和MAPK信号通路有关.
- 鉴定出酸和贝尔根因是主要的贡献化合物.
结论:
- 阿斯蒂尔博伊德 (Astiboides tabularis) 根的乙烯酸分离物具有显著的抗氧化和抗炎性质.
- 这些效应通过激素清除和抑制炎症信号通路,特别是NF-κB和MAPK进行调解.
- 鉴定到的化合物,酸和伯根因,可能有助于观察到的治疗潜力,支持使用A.tabularis用于炎症条件.
关键词:
这种植物叫阿斯蒂尔博伊德 (Astilboides tabularis) (Hemsl.) 英语:英格兰.在NF-κB/MAPK信号传输中.抗炎机制 抗炎机制 抗炎机制抗氧化剂活性 抗氧化剂活性在Bergenin的Bergenin里面酸是一种酸.更多相关视频
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