从乳,葡萄和杏仁核油中批量和连续的脂酶催化生产饮食结构脂质
Şuheda Akbaş1,2, Natália M Osório3, Suzana Ferreira-Dias2,4
1Department of Food Engineering, Faculty of Engineering, Talas Campus, Erciyes University, 38039 Kayseri, Türkiye.
Molecules (Basel, Switzerland)
|May 14, 2025
概括
结构性脂质 (SL) 是通过酶反应从植物油中制成的. 高产量的MLM型SLs通过利化和酸解来实现,在连续过程中显示出稳定的脂酶活性.
科学领域:
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
- 可持续化学 可持续化学
背景情况:
- 循环生物经济强调减少废物和利用副产品.
- 结构性脂质 (SLs) 对健康有好处,可以从各种脂质来源产生.
- 农业工业副产品和非传统油是可持续脂质生产的宝贵原料.
研究的目的:
- 从乳,葡萄和杏仁核油中生产低热量,MLM型结构性脂质 (SL).
- 在无溶剂介质中研究SL合成的酶性酸解和兴化.
- 在批量和连续反应器系统中评估固定脂质酶的稳定性和效率.
主要方法:
- 脂酶催化酸解与酸和兴趣化与乙基酸.
- 在批量反应中使用固定的 *Rhizomucor miehei* 脂酶 (Lipozyme RM).
- 在一个包装床生物反应器中,使用固定的*Thermomyces lanuginosus*脂酶 (Lipozyme TL IM) 持续生产SLs.
主要成果:
- 在批量反应中,在6小时的兴趣化后,新TAG (SL) 的高产量在61%至63%之间得到.
- 通过不同反应时间的酸分解,通过不同反应时间获得了64.7% (葡萄种子),56.1% (乳) 和69.7% (杏仁核) 的最大SL产量.
- 150小时的连续运行显示出稳定的脂酶性能,平均SL产量为79.2%±4.1 (兴化) 和61.5%±5.91 (酸解).
结论:
- 酶性兴化和酸解是从植物油中生产MLM类型结构性脂质的有效方法.
- 固定化脂酶在SL合成的批量和连续过程中都表现出高的稳定性和效率.
- 这种方法支持循环生物经济框架内的可持续脂质生产,利用非传统的石油来源.
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