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在播种和结合阶段,冬季小麦化等位基因和结耐受性

Fangfang Liu^1,2, Wenxin Cao^1,2, Qiqi Zhang^1,2

¹Crop Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230001, China.

Plants (Basel, Switzerland)

|May 14, 2025

概括

冬季小麦的冷耐受性在播种和结合阶段的基因调节不同. 播种阶段的耐受性与本地化基因组合,特别是VRN-D1有很强的相关性,而结合阶段的耐受性没有显示这种关联.

科学领域:

植物遗传学植物遗传学
农作物科学农作物科学
农业生物技术农业生物技术

背景情况:

冬季小麦 (Triticum aestivum L.) 需要花开,这是VRN基因调节的过程.
结耐受性是冬季小麦生存和产量的关键特征,但它在整个发展阶段的遗传基础是复杂的.
了解本土化基因与耐性之间的关系,可以帮助开发更坚固的小麦品种.

研究的目的:

为了研究白色化基因 (VRN-A1,VRN-B1,VRN-D1,VRN-B3) 的等位基因变异与冬季小麦的冷耐受性之间的联系.
为了确定冷耐受性是否由VRN基因在播种和结合阶段进行特定阶段的调节.
为开发用于冬季小麦育种中标记辅助选择的分子标记物提供基础,以提高结耐受性.

主要方法:

通过实地测试,通过播种阶段评估了435个冬季小麦加入的结耐受性.
在结合阶段使用气候室测试评估了192个冬季小麦加入的结耐受性.
对VRN-A1,VRN-B1,VRN-D1和VRN-B3位点的基因型小麦连接使用异位基因特定标记物.

主要成果:

在小麦加入中观察到结耐受性的显著差异.
在幼苗和结合阶段的冷耐受性之间没有发现显著的相关性.
幼苗阶段的冷耐受性与VRN基因等位基因组合 (p <0.05) 有着强烈的关联,需要所有衰退等位基因 (vrn-A1,vrn-B1,vrn-D1,vrn-B3) 才能获得强烈的耐受性.
Vrn-D1是最常见的主导等位基因;主导的Vrn-A1和Vrn-B3等位基因缺席.
当vrn-A1和vrn-B1等位基因是衰退的时,VRN-D1标记物对选幼苗阶段冷耐受性的有效性得到证实.

结论:

冬季小麦的冷耐受性通过本地化基因表现出特定阶段的遗传调节.
VRN基因等位基因组合对于幼苗阶段的冷耐受性至关重要,特定的衰退等位基因是必不可少的.
在冬季小麦育种计划中,VRN-D1标记物有潜力用于标记物辅助的选择,以提高冬季小麦育种计划中幼苗阶段的冷耐受性.

关键词:

结耐受度是可以容忍的.连接阶段是连接阶段.播种阶段播种阶段基因的民族化基因的民族化冬季小麦冬季小麦

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