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基本RNA测序数据处理和转录组分析指南

Rowayna Shouib1, Gary Eitzen2, Rineke Steenbergen2

  • 1Faculty of Biotechnology, October University for Modern Sciences and Arts (MSA), Giza, Egypt.

Bio-protocol
|May 14, 2025
PubMed
概括

本指南为研究人员简化了RNA测序 (RNA-Seq) 数据分析. 它提供了一个初学者友好的协议来分析下一代测序 (NGS) 数据,识别基因表达,并可视化结果.

关键词:
生物信息学是一种生物信息学.下一代测序 (NGS) 是指下一代的测序.在RNA-Seqq.转录组分析 转录组分析它们是mRNARNA.

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
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