Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

9.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.7K
Sanger Sequencing01:57

Sanger Sequencing

751.5K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
751.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A Simple, Cost-Effective, High-Throughput Method for Measuring Chromatin Accessibility and Gene Expression in Single Nuclei.

bioRxiv : the preprint server for biology·2026
Same author

Epigenetic landscape, key transcriptional regulators, and in vivo identification of human Tr1 cells.

Science advances·2026
Same author

Intrinsic promoter responsiveness dictates sensitivity to transcriptional activation by enhancers.

bioRxiv : the preprint server for biology·2026
Same author

An emergent disease-associated motor neuron state precedes cell death in ALS.

Cell·2026
Same author

Ribo-Tweezer: Rapid removal of ribosomal proteins reveals additional layers of post-transcriptional gene regulation.

Molecular cell·2026
Same author

Prediction and functional interpretation of inter-chromosomal genome architecture from DNA sequence with TwinC.

Nature communications·2026

相关实验视频

Updated: May 16, 2025

ATAC-Seq Optimization for Cancer Epigenetics Research
07:13

ATAC-Seq Optimization for Cancer Epigenetics Research

Published on: June 30, 2022

4.1K

用KAS-ATAC测序映射同时可访问和ssDNA含有的基因组.

Georgi K Marinov1, William J Greenleaf1,2,3,4,5

  • 1Department of Genetics, School of Medicine, Stanford University, Stanford, CA, USA.

Bio-protocol
|May 14, 2025
PubMed
概括

该KAS-ATAC测试映射的DNA片段,既是物理上可访问,并包含单链DNA (ssDNA) 泡. 这种方法有助于理解基因调节,通过识别活性cis调节元素和RNA聚合酶活性.

关键词:
ATAC-seqq 的使用情况.染色是一种染色素.染色体的可访问性 染色体的可访问性这是一个KAS-ATAC.这就是KAS-seqq.转录 转录 转录 转录这就是 ssDNADNA.

更多相关视频

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

5.7K
Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
09:08

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

17.9K

相关实验视频

Last Updated: May 16, 2025

ATAC-Seq Optimization for Cancer Epigenetics Research
07:13

ATAC-Seq Optimization for Cancer Epigenetics Research

Published on: June 30, 2022

4.1K
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

5.7K
Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
09:08

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

17.9K

科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 基因调节涉及 cis 调节元件 (cRE) 和 RNA 聚合酶活性.
  • 活跃的cRE通常是核细胞体枯竭的,并由转录因子结合.
  • RNA聚合酶活动可以创建单链DNA (ssDNA) 结构.

研究的目的:

  • 提出KAS-ATAC测试的详细方案.
  • 描述KAS-ATAC数据集的基本处理.
  • 讨论成功应用KAS-ATAC的关键考虑因素.

主要方法:

  • KAS-ATAC测试捕获具有物理可访问性和ssDNA泡的DNA片段.
  • 涉及N3-基托克萨尔标记和原生染色素的转换.
  • 包括生物标记的DNA片段拉出和库生成.

主要成果:

  • 该协议使得同时可访问和包含ssDNA的DNA片段的映射成为可能.
  • 详细的步骤为N3-基托克萨尔标记和染色素转换提供.
  • 描述了DNA片段提取和库准备的方法.

结论:

  • KAS-ATAC是研究基因调节过程的一个有价值的方法.
  • 该协议有助于调查活跃的cRE和RNA聚合酶与DNA的关联.
  • 成功应用需要仔细考虑协议执行和数据处理.