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一个新的关闭光NIR探针用于低酸及其应用

Ayten Ibrahimova¹, Ayşe Nur Önem¹, Mehmet Altun²

¹Division of Analytical Chemistry, Department of Chemistry, Faculty of Engineering, Istanbul University-Cerrahpaşa, İstanbul, Turkiye.

Turkish journal of chemistry

|May 14, 2025

概括

我们开发了一种新的,快速和灵敏的计方法来检测低酸 (HOCl),这是免疫系统的关键分子. 这种方法使用近红外光探头NIR-QBH来准确检测生物样本中的HOCl.

科学领域:

生物化学生物化学
分析化学分析化学
免疫学免疫学免疫学

背景情况:

酸 (HOCl) 是生理过程中,特别是人类免疫系统中重要的非激进氧化剂.
准确检测HOCl对于了解它在生物系统中的作用至关重要.

研究的目的:

引入一种新,快速,高效的度检测方法来检测HOCl.
使用基于近红外 (NIR) 的光探测器,NIR-QBH,用于敏感和稳定的HOCl传感.

主要方法:

开发一种使用NIR-QBH探针进行度测试的方法,该探针含有烯酸C=C键.
基于HOCl诱导的C=C键在NIR-QBH中的氧化,导致光损失的检测机制.
使用谷氨 (GSH) 作为参考对HOCl清理活动的评估.

主要成果:

该NIR-QBH探测器在660nm (λex = 618nm) 呈现强烈的NIR辐射和高灵敏度.
该方法实现了0.23μM的低检测极限,快速响应时间为4分钟.
量化了GSH的HOCl清理活性,其IC50值为8.97μM.
在胎牛血清 (FBS) 和水溶液中检测HOCl的试验的成功应用.

结论:

开发的度法为HOCl检测提供了灵敏,快速和高效的手段.
在复杂的生物矩阵中,NIR-QBH探测器是量化HOCl的宝贵工具.
这种测定有助于进一步研究HOCl.Cl的生理作用.

关键词:

这是GSHGSH.低酸是什么?高酸是什么?在NIR中,NIR是NIR.细胞毒性细胞毒性化探测器的化探测器

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