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尿液细胞外囊中的EIF2S1作为膀癌的新型诊断标记物

Eisuke Tomiyama¹, Kazutoshi Fujita^1,2, Kyosuke Matsuzaki¹

¹Department of Urology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.

Cancer medicine

|May 14, 2025

概括

尿液细胞外囊泡 (uEVs) 在膀癌 (BCa) 检测方面表现有前途. 在UEV中发现的EV-EIF2S1作为BCa的新型诊断标志物,对细胞生长至关重要.

科学领域:

蛋白质组学是指蛋白质组学.
生物标志物发现发现
癌症的诊断癌症的诊断

背景情况:

尿液细胞外囊泡 (UEV) 是由膀癌 (BCa) 细胞分泌的.
uEV有潜力成为发现BCa.诊断生物标志物的来源.

研究的目的:

探索和验证膀癌 (BCa) 基于尿液细胞外囊泡 (uEV) 的诊断标记物.
专注于细胞质EV蛋白用于BCa检测.

主要方法:

来自BCa患者和健康个体的UEVs的蛋白质组分析 (枪支和准).
使用LC-MS/MS和SRM/MRM识别和验证尿液中细胞质EV蛋白升高.

主要成果:

17 细胞质EV蛋白在BCa患者的尿液中显著升高.
EV-EIF2S1成为一个有前途的标志物,区分BCa和非BCa血 (AUC:0.92).
EIF2S1敲击抑制了BCa细胞增殖,诱导了细胞循环停止和细胞亡.

结论:

EV-EIF2S1被确定为一种新的基于尿液细胞外囊泡 (uEV) 的膀癌 (BCa) 诊断标志物.
EV-EIF2S1在膀癌 (BCa) 细胞生长和存活方面发挥着重要作用.

关键词:

膀癌:膀癌是一种癌症.细胞质蛋白质的细胞质蛋白质.细胞外囊泡中的细胞外囊泡.液体活检活检液体活检蛋白质组学蛋白质组学尿路标记物尿路标记物

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