双氧-无氧共同培养使得在呼吸道上皮层接口上直接研究无氧生物与宿主相互作用
Patrick J Moore1, Kayla Hoffman1, Sara Ahmed2
1Department of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA.
一种新的双氧-无氧培养 (DOAC) 方法使得无氧细菌和呼吸道细胞的研究成为可能. 这种方法揭示了Fusobacterium nucleatum如何改变其新陈代谢和毒性,以及呼吸道细胞如何反应,为呼吸道疾病提供了洞察力.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 病理生理学 病理生理学
背景情况:
- 厌氧细菌与呼吸道疾病有关,但由于建模的局限性,它们的作用尚不清楚.
- 无气体和宿主细胞之间相冲突的氧气需求阻碍了体外研究.
研究的目的:
- 开发和验证双氧-无氧培养 (DOAC) 平台,用于研究宿主-无氧生物相互作用.
- 为了研究呼吸道上皮细胞和*Fusobacterium nucleatum*之间的相互转录反应.
主要方法:
- 开发了一种双氧-无氧培养 (DOAC) 系统,以保持差异氧度梯度.
- 利用批量和单细胞RNA测序来分析细菌和宿主细胞中的基因表达.
- 与呼吸道上皮细胞共培养 * Fusobacterium nucleatum * 大约48小时.
主要成果:
- DOAC成功地支持了对氧气敏感的厌氧生物和需要氧气的宿主细胞的共同培养.
- *F. nucleatum*在表皮细胞殖民时表现出与毒性和代谢相关的基因表达变化.
- 空气道上皮细胞显示了细胞类型特定的反应,包括炎症和癌症相关途径的上调.
结论:
- DOAC平台非常适合研究体内的不同部位的无氧体与宿主相互作用.
- 揭示了新的机械洞察力,了解 *F. nucleatum* 如何与呼吸道上皮相互作用.
- 为了解无氧微生物群在呼吸道疾病病理生理学中的作用提供了基础.
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