Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Proteomics01:33

Proteomics

7.3K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.3K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Rapid Peptide Mapping of Monoclonal Antibodies with Direct Infusion Mass Spectrometry.

bioRxiv : the preprint server for biology·2026
Same author

SLX4IP limits replication stress globally and at ALT telomeres.

The EMBO journal·2026
Same author

Primate lineage specification requires suppression of Alu hyperediting.

bioRxiv : the preprint server for biology·2026
Same author

Regarding Emitter Positioning for Nanoflow Electrospray Ionization with a High-Capacity Inlet Capillary.

Journal of the American Society for Mass Spectrometry·2026
Same author

Proteomic and Lipidomic Atlas of Gut-Associated Lymph and Venous Depots in Female Piglets.

Arteriosclerosis, thrombosis, and vascular biology·2026
Same author

Atlas of lysine acetylation in the mouse.

bioRxiv : the preprint server for biology·2026
Same journal

From Method-Defined Signals to Reference Measurement Procedures: Two Decades of Mass Spectrometry-Based ProGRP Quantification.

Journal of proteome research·2026
Same journal

Proteomic Profiling of Extracellular Vesicle-Enriched Plasma Using Mag-Net for Biomarker Discovery in Pancreatic Ductal Adenocarcinoma.

Journal of proteome research·2026
Same journal

Computationally Efficient Bayesian Estimation of Graphical Networks for Omics Data.

Journal of proteome research·2026
Same journal

Hierarchy of MS-Based Evidence.

Journal of proteome research·2026
Same journal

Proteomic Profiling of Exosomes from HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinoma: Selective Cargo Packaging.

Journal of proteome research·2026
Same journal

Proteomic Analysis Identifies ATE1-Dependent Arginylation Dysregulation across Meningioma Grades.

Journal of proteome research·2026
查看所有相关文章

相关实验视频

Updated: Jun 14, 2025

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
07:12

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

Published on: April 24, 2020

10.0K

技术评估等离子体蛋白质组技术的技术评估

William F Beimers1, Katherine A Overmyer1,2,3, Pavel Sinitcyn2,4,5

  • 1Department of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin 53506, United States.

Journal of proteome research
|May 14, 2025
PubMed
概括
此摘要是机器生成的。

这项研究评估了六种用于生物医学研究的血蛋白质组学技术. 预见者蛋白质 XT 提供了最大的蛋白质深度和可量化的蛋白质,尽管所有方法在检测癌症生物标志物方面都显示出差异.

关键词:
这就是LC-MS.这是一个磁网.奥林克·奥林克·奥林克·奥林克·奥林克·奥林克·奥林克·奥林克·奥林克·奥林克·奥林克·奥林克·奥林克在PreOmics的基础上,PreOmics是最重要的.预见者 预见者 预见者质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量方法比较方法比较血等离子体是什么?蛋白质组学 蛋白质组学

更多相关视频

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies
05:30

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Published on: January 31, 2025

350
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.9K

相关实验视频

Last Updated: Jun 14, 2025

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
07:12

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

Published on: April 24, 2020

10.0K
Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies
05:30

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Published on: January 31, 2025

350
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.9K

科学领域:

  • 生物医学研究的研究.
  • 蛋白质组学是指蛋白质组学.
  • 分析化学是一种分析化学.

背景情况:

  • 血蛋白质组技术对于生物医学研究和快速发展至关重要.
  • 评估这些技术对于选择适合各种应用的方法至关重要.

研究的目的:

  • 对六种突出的等离子体蛋白质组学技术进行技术评估.
  • 根据蛋白质深度,可复制性,线性,脂质干扰耐受性和检测/定量限值来比较它们的性能.
  • 评估它们在区分健康和非小细胞肺癌 (NSCLC) 患者样本中的适用性.

主要方法:

  • 评价了六种等离子体蛋白质组技术:未富化 (Neat),酸耗尽,PreOmics ENRICHplus,Mag-Net,Seer Proteograph XT和Olink Explore HT. 这些技术包括:未富化 (Neat),酸耗尽,PreOmics ENRICHplus,Mag-Net,Seer Proteograph XT和Olink Explore HT.
  • 性能指标包括蛋白质组深度,可重现性,线性,脂质干扰耐受性和检测/定量限度 (LOD/LOQ).
  • 总共进行了618次液体染色学-并联质谱 (LC-MS/MS) 实验和93次Olink Explore HT测试. 使用非小细胞肺癌 (NSCLC) 队列来测试临床适用性.

主要成果:

  • 预见者蛋白质 XT 实现了最高的蛋白质深度 (∼4500 种蛋白质) 和可量化的蛋白质数量 (LOD: 4407,LOQ: 2696).
  • 奥林克探索HT检测到约2600种蛋白质,其中2002种具有LOD,1883种具有LOQ.
  • 尼特,Mag-Net,赛尔和奥林克表现出良好的可重复性,而PreOmics和Acid显示出更高的变化性. 所有MS方法都表现出与尖端C反应蛋白 (CRP) 具有良好的线性.
  • 所有六种方法都在健康和NSCLC样本之间检测出差异丰富的蛋白质,但在特定的蛋白质中观察到显著的差异.

结论:

  • 在评估的方法中,Seer Proteograph XT和Olink Explore HT提供了卓越的蛋白质深度和量化能力.
  • 虽然所有测试的技术都能检测到NSCLC中的差异蛋白质丰度,但存在特定方法的偏差,影响了重要的生物标志物的识别.
  • 选择等离子体蛋白质组学技术显著影响蛋白质组学研究的结果,特别是对于NSCLC等疾病的生物标志物发现.