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普拉梅可以增强精子与卵子的结合,并调节牛胚胎发生的表观遗传动力学

Chandlar Kern¹, Wan-Sheng Liu²

¹Department of Animal Science, Center for Reproductive Biology and Health (CRBH), College of Agricultural Sciences, The Pennsylvania State University, University Park, PA, 16802, USA.

Cell and tissue research

|May 14, 2025

概括

牛的不孕可能与PRAMEY基因有关. 抑制PRAMEY改善了精子与卵子的结合,并在早期牛胚胎中改变了DNA和基因素甲基化.

科学领域:

生殖生物学生殖生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
动物遗传学动物遗传学

背景情况:

不孕不育和不孕不育是牛繁殖的主要问题.
遗传因素,包括像PRAMEY这样的Y相关基因,都与生殖挑战有关.
PRAMEY是一种参与生殖细胞形成,受精和胚胎发育的候选基因.

研究的目的:

为了研究牛PRAMEY基因在精子-卵结合中的作用.
评估PRAMEY对精子体完整性的影响.
检查PRAMEY对牛受精和早期胚胎发生过程中的表观遗传修饰的影响.

主要方法:

在体外受精 (IVF) 使用用PRAMEY抗体或控制IgG治疗的牛精子.
在受精后2,4和6小时 (hpf) 评估精卵结合和体完整性.
通过免疫光学分析DNA (5-methylcytosine) 和histon (H3K9me3,H3K27me3) 在胚胎和胚胎中的甲基化.

主要成果:

在所有时间点中,Pramey抗体治疗显著增加了精子与卵子的结合,特别是在6 hpf时.
在治疗组之间没有观察到精子体完整性的显著差异.
在PRAMEY抑制后,在父亲和母亲的前核中减少了DNA甲基化 (5-mC),并在早期胚胎中减少了H3K27me3基因组甲基化.

结论:

在牛中,PRAMEY在调节精子-卵结合和表观遗传修饰方面发挥作用.
抑制PRAMEY增强了精子-卵子相互作用,并影响了DNA和基因素甲基化模式.
这些发现凸显了PRAMEY在牛受精和早期胚胎发育中的潜在意义.

关键词:

牛群是牛群,牛群是牛群,牛群是牛群.通过DNA甲基化.希斯甲基化希斯甲基化普莱姆尼是什么意思普莱姆尼精子与卵子结合在一起.

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