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自组装固定和金属多网络封装T4菌体上的β-银酸酶,以提高生物催化性能

Dan Wu¹, Sulaiman Khan¹, Shujie Zhang¹

¹Advanced Biomaterials and Tissue Engineering Center, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, Hubei Province, China.

Applied biochemistry and biotechnology

|May 14, 2025

概括

在T4菌上酶固定,通过金属聚烯网络涂层增强,显著提高了酶的稳定性和对恶劣条件的抵抗力,提供了一个有前途的生物催化剂解决方案.

科学领域:

生物催化剂是一种生物催化剂.
酶工程是什么? 酶工程是什么?
材料科学材料科学材料科学

背景情况:

酶是重要的生物催化剂,但在具有挑战性的条件下容易变质,增加运营成本.
在纳米颗粒和病毒等载体上酶固定是一种提高稳定性的策略.
T4菌体提供了一个经济高效和稳定的平台,用于酶固定.

研究的目的:

为了将Soc-β-galactosidase (Soc-β-gal) 固定在T4菌上.
为了提高固定酶的稳定性,使用金属多网络 (MPN) 涂层.
评估修改酶的生物化学特性和稳定性.

主要方法:

在T4菌上Soc-β-gal的亲和固定.
用金属多网络 (MPN) 涂层封装固定酶.
对酶活性和在热,紫外线和蛋白质分解应激下稳定的比较分析.

主要成果:

在50°C下6个小时后,固定化的酶β-gal T4的活性保持在85%以上,明显高于自由的Soc-β-gal (40.63%).
与未涂层的固定酶 (10.21%) 和自由酶 (7.23%) 相比,MPN涂层的酶 (β-gal T4@TA-Ti) 显示出优越的紫外线稳定性 (92.88%的活性保留).
MPN涂层提高了对蛋白质分解的抗性,β-gal T4@TA-Ti对蛋白酶K保持了9.48%的活性,而β-gal T4.4的活性为4.62%.

结论:

在T4菌上酶固定增强了酶的稳定性.
该MPN涂层进一步提高了对UV辐射和蛋白质溶解等环境压力因素的酶耐药性.
这种方法具有强大的生物催化剂应用的巨大潜力.

关键词:

酶不移动化酶不移动化金属聚烯网络 - 金属聚烯网络体显示器是体显示器.在T4菌体.这就是β-Galactosidase.

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