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在小鼠克隆过程中通过JNJ-770662121的激活后治疗来增强胚胎和全程发育

Hyo-Gu Kang¹, Min Ju Kim², Ji Hyeon Yun³

¹Futuristic Animal Resource & Research Center (FARRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Republic of Korea; Department of Animal Science and Biotechnology, College of Agriculture and Life Science, Chungnam National University, Daejeon, Republic of Korea.

|May 14, 2025

概括

JNJ-7706621 (JNJ) 通过增强细胞骨完整性和染色体稳定性,显著改善了体细胞核转移 (SCNT) 的小鼠胚胎发育. 与传统方法相比,这种新的治疗方法提供了更好的植入前发育和全程成功率.

科学领域:

生殖生物学生殖生物学
发展生物学发展生物学
细胞生物学细胞生物学

背景情况:

体细胞核转移 (SCNT) 是动物克隆的一个关键技术.
由SCNT衍生的胚胎通常比自然受孕的胚胎具有较低的发育潜力.
目前的活化后治疗方法,如细胞素B (CB),在改善SCNT胚胎发育方面存在局限性.

研究的目的:

评估JNJ-7706621 (JNJ) 作为一种潜在的后激活治疗,以取代小鼠SCNT中的细胞素B (CB).
确定JNJ的最佳度,以改善SCNT胚胎发育.
评估JNJ对胚胎发育,细胞活力和染色体稳定性的影响.

主要方法:

帕氏基因活化 (PA) 鼠标胚胎被用不同度的JNJ (1,10,50μM) 或CB (5μg/mL) 治疗.
随后,SCNT小鼠胚胎用最佳的JNJ度 (10微米) 或CB治疗.
分析了胚胎发育,细胞计数 (总数,ICM,托菲科德尔姆),细胞亡,F-actin,突蛋白,螺旋体形态,DNA损伤,植入和活产率.

主要成果:

与CB (39.9%) 相比,10μM JNJ治疗显著改善了囊胞形成率 (61.4%).
JNJ治疗增加了总细胞数量,内细胞质量和肌体内细胞,同时减少了亡细胞.
与CB相比,JNJ显著减少了异常的细胞骨架结构 (F-actin,tubulin),异常的,胚芽细胞碎片化和DNA损伤,导致更高的植入率 (68.3%) 和活产率 (10.9%).

结论:

在SCNT小鼠胚胎中,JNJ-7706621有效增强细胞骨完整性和染色体稳定性.
在SCNT中,JNJ作为细胞素B的优质替代品,用于SCNT的活化后治疗.
这项研究表明,JNJ有潜力改善SCNT应用的植入前开发和长期成功.

关键词:

动氨酸丝是指一种氨酸丝.染色体是什么染色体是什么染色体是什么在体外培养培养.JNJ-770662121是什么意思?JNJ-7706621是什么意思鼠标胚胎是如何形成的实体细胞的核转移转移螺旋的螺旋是一个螺旋.

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