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TCREMP:一种生物信息管道,可以有效地嵌入来自免疫库的T细胞受体序列和单细胞测序数据的T细胞受体序列

Yulia Kremlyakova¹, Elizaveta K Vlasova², Daniil Luppov³

¹Institute of Translational Medicine, Pirogov Russian National Research Medical University, Moscow, Russia.

Journal of molecular biology

|May 14, 2025

概括

对T细胞受体 (TCR) 测序分析具有挑战性. TCREMP将TCR序列数字化,使适应性免疫研究能够有效区分抗原特异性T细胞群.

科学领域:

免疫学免疫学免疫学
生物信息学是一种生物信息学.
计算生物学计算生物学

背景情况:

T淋巴细胞利用T细胞受体 (TCR) 识别细胞表面的抗原,区分健康细胞和异常细胞.
高通量TCR的测序揭示了免疫反应,但对数据分析和抗原特异性追踪提出了生物信息学挑战.
了解TCR曲目多样性对于适应性免疫研究至关重要.

研究的目的:

开发一种新的计算管道,TCREMP (TCR通过原型嵌入),用于分析TCR氨基酸序列.
创建一种基于与常见参考序列相似性的数字化TCR序列的方法.
为了促进比较分析和改善TCR谱系研究中的抗原特异性预测.

主要方法:

TCREMP管道通过基于与常见原型的相似性嵌入TCR氨基酸序列来数字化它们.
该方法用于分析TCR目录测序数据,包括单细胞和MHC多元分类样本.
进行了比较分析,以评估管道在区分免疫细胞群中的有效性.

主要成果:

TCREMP嵌入有效地区分不同的抗原特异性T细胞群.
该管道证明了分析单细胞和批量TCR测序数据的实用性.
该方法为比较TCR曲目分析提供了有价值的参考点.

结论:

TCREMP提供了一种高效的生物信息解决方案,用于处理复杂的TCR测序数据.
该管道有助于解码抗原特异性和理解适应性免疫.
TCREMP通过提供一个强大的方法来分析TCR的回应,从而促进免疫反应的研究.

关键词:

这种T细胞受体是T细胞受体.抗原是一种抗原.嵌入式嵌入式嵌入式测序的测序是指测序的测序.一个单细胞的单细胞.

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