层次的糖解路径控制了人类肠道中的碳水化合物利用调节器Bacteroides
Seth G Kabonick1,2,3, Kamalesh Verma1,2,3, Jennifer L Modesto1,2,3
1Penn State College of Medicine, Hershey, PA, USA.
Nature communications
|May 14, 2025
概括
像葡萄糖和果糖这样的简单糖会抑制肠道细菌中的Cur调节剂,破坏宿主健康. 这种抑制需要依赖ATP的果糖-1,6-双酸盐 (FBP) 合成,影响细菌类的物种.
科学领域:
- 微生物学 微生物学
- 肠道微生物组研究研究
- 代谢调节 代谢调节 代谢调节
背景情况:
- 人类高含简单糖 (葡萄糖,果糖) 的饮食与肠道微生物组失调有关.
- 在Bacteroides物种中,碳水化合物利用调节器 (Cur) 控制关键功能,包括宿主相互作用.
- 众所周知,简单糖会抑制Cur活性,但确切的机制尚不清楚.
研究的目的:
- 阐明简单糖抑制哺乳动物肠道Cur转录因子的分子机制.
- 研究ATP依赖的果糖-1,6-双酸盐 (FBP) 合成在糖的Cur调节中的作用.
- 了解Cur抑制对Bacteroides健康和宿主有益产品的影响.
主要方法:
- 研究了葡萄糖和果糖对两种细菌类的Cur活性的影响.
- 使用遗传和生化方法评估了依赖ATP的FBP合成对于糖诱导的Cur抑制的必要性.
- 在具有Cur和没有Cur的条件下比较细菌生长和健康状况,评估酸盐 (PPi) 依赖酶的作用.
主要成果:
- 以ATP为依赖的果糖-1,6-双酸盐 (FBP) 合成对于葡萄糖或果糖来抑制Bacteroides中的Cur活性至关重要.
- 这种依赖ATP的FBP合成对于细菌生长是不可或缺的,因为另一种依赖酸盐 (PPi) 的酶可以履行这一作用.
- 虽然对于肠道中的Cur调节是必要的,但当Cur缺少时,依赖ATP的FBP合成不会增强适应性,这表明PPi足以进行糖解.
结论:
- 简单糖通过依赖ATP的FBP合成途径抑制Bacteroides中的Cur.
- 这种由糖诱导的抑制破坏了Bacteroides的健康状况,并减少了对宿主有益的代谢物的产生.
- 饮食中的糖摄入量可以通过Cur介导的机制对肠道微生物组的组成和功能产生负面影响.
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