marsupial 和 eutherian 胚胎中的不同DNA甲基化动态
Bryony J Leeke1,2,3, Wazeer Varsally4, Sugako Ogushi4
1Sex Chromosome Biology Laboratory, The Francis Crick Institute, London, UK. b.leeke@lms.mrc.ac.uk.
Nature
|May 14, 2025
概括
marsupials 和 eutherians 的 DNA 甲基化重编程不同. 子胚胎的DNA甲基化时间更长,这表明哺乳动物的发育策略不同.
科学领域:
- 发育生物学
- 表观遗传学
- 比较基因组学
背景情况:
- 哺乳动物的发育传统上假定基因组范围内的DNA甲基化擦除对于胚胎发生是必不可少的,基于eutherian模型.
- 这些DNA甲基化重编程事件在多种哺乳动物群体中的保存仍然在很大程度上未被探索.
研究的目的:
- 在一个有袋动物 (Monodelphis domestica) 中研究DNA甲基化重编程,以了解其在哺乳动物中的保存.
- 为了与已建立的eutherian模型进行DNA甲基化模式的比较.
主要方法:
- 基准分辨率的DNA甲基化图的生成.
- 在野子,胚胎 (分裂阶段,胚泡) 和成年组织中的DNA甲基化分析.
- 与现有的eutherian DNA甲基化数据进行比较分析.
主要成果:
- marsupial DNA 的甲基化重编程与 eutherians 有显著差异; 游戏甲基化差异不那么明显.
- 胚胎在裂变阶段保持高甲基化,而胚胎细胞中的脱甲基化在表细胞中是暂时的,但在体中是持续的.
- 不活跃的X染色体的全球DNA低甲基化发生在野胚胎生成过程中,与eutherians相反.
结论:
- 在胚胎发生过程中,Eutherian和有袋动物使用不同的DNA脱甲基化策略.
- 在体中持续的DNA低甲基化表明在哺乳动物的胎盘进化中发挥了保留作用.
- 鉴定了涉及RSX母体DNA甲基化的印记X失活的潜在机制.
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