相关实验视频

Updated: May 17, 2025

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

在氧气不足的慢性髓性白血病细胞中,BCR::ABL1的表达通过CD36介导的脂肪酸吸收通过谷氨酸受到调节

Caterina Mancini¹, Giulio Menegazzi², Silvia Peppicelli²

¹Department of Experimental and Clinical Medicine, Università degli Studi di Firenze, Florence, Italy.

Cancer cell international

|May 14, 2025

概括

脂肪酸代谢对于慢性髓性白血病 (CML) 干细胞生存至关重要,特别是在低氧条件下. 谷氨酸通过驱动脂肪酸的吸收和抑制BCR::ABL1上蛋白质来支持CML细胞的存活.

科学领域:

癌症生物学癌症生物学
代谢调节代谢调节代谢调节
血液学恶性瘤血液学恶性瘤

背景情况:

慢性髓性白血病 (CML) 的进展受到微环境营养素,如葡萄糖 (Glc) 和谷氨酸 (Gln) 的影响.
这些营养物质调节癌细胞的增殖,活力和BCR::ABL1coprotein的表达.

研究的目的:

在低氧条件下研究葡萄糖和谷氨酸在CML细胞代谢中的作用.
阐明脂肪酸代谢对白血病干细胞 (LSC) 存活率和BCR::ABL1调节在CML中的贡献.

主要方法:

在低氧条件下培养CML细胞系 (K562,KCL22),营养缺乏.
进行了代谢分析,脂质滴滴分析和BCR::ABL1和脂肪酸标记物的评估.
使用脂肪细胞和抑制脂肪酸吸收的共同培养实验被用于研究CML-脂肪细胞相互作用和LSC潜力.

主要成果:

葡萄糖在低氧条件下对CML细胞生长至关重要,而谷氨酸单独无效,但可以增强糖解并抑制氧化呼吸.
在缺乏葡萄糖的情况下,脂肪酸代谢变得至关重要,脂肪滴积累增加.
谷氨酸促进CD36介导的脂肪酸吸收,抑制BCR::ABL1并提高细胞存活率.

结论:

脂肪酸代谢在调节CML中白血病干细胞区中发挥着中心作用.
谷氨酸在代谢压力下对CML细胞存活至关重要,在条件改善时启动细胞扩张.

关键词:

BCR:ABL1 的意思是慢性骨髓性白血病慢性骨髓性白血病脂肪酸是一种脂肪酸.氧气过低是因为缺氧.

更多相关视频

Assessment of Glutamine as a Fuel Source for Alveolar Macrophages Exposed to Chronic Ethanol Using an Extracellular Flux Bioanalyzer

Assessment of Glutamine as a Fuel Source for Alveolar Macrophages Exposed to Chronic Ethanol Using an Extracellular Flux Bioanalyzer

Published on: November 15, 2024

Assessment of the Metabolic Profile of Primary Leukemia Cells

Published on: November 21, 2018

相关概念视频

Regulation of Nuclear Protein Sorting

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...