来自1,3,5和8系的两种复合PRRSV-2菌株的遗传特征和致病性在中国出现了
Chunhua Wei1,2,3, Chen Liu1,4, Guangsong Chen1
1College of Life Sciences, Longyan University, Longyan, Fujian, 364012, China.
BMC veterinary research
|May 14, 2025
概括
新的重组猪生殖和呼吸系统综合征病毒 (PRRSV) - 2菌株在中国出现. FJLIUY-2017在小猪中显示出更高的致病性,导致20%的死亡率,与PRRSV2/CN/G8/2018不同.
科学领域:
- 兽医病毒学 兽医病毒学
- 动物病理学 动物病理学
背景情况:
- 猪生殖和呼吸系统综合征病毒 (PRRSV) 对猪产业构成重大经济威胁.
- 涉及NADC30类PRRSV和本地中国菌株的复杂重组事件加速了PRRSV的进化.
- 最近,来自多个血统的新型重组PRRSV-2菌株在中国出现,具有有限的致病性数据.
研究的目的:
- 分析两种新型重组PRRSV-2菌株FJLIUY2017和PRRSV2/CN/G8/2018在小猪中的致病性.
- 了解这些新出现的PRRSV菌株的遗传特征和进化起源.
主要方法:
- 隔离和PRRSV FJLIUY2017和PRRSV2/CN/G8/2018的全长基因组测序.
- 使用DNASTAR 7.0,MEGA 7.0,RDP V4.10和SIMPLOT软件进行了家族遗传和重组分析.
- 无PRRSV小猪的鼻内注射,然后进行日常临床监测,病毒载量量化 (IFA),抗体水平评估 (ELISA) 和粗/微观肺损伤评分.
主要成果:
- FJLIUY2017和PRRSV2/CN/G8/2018与现有的GenBank PRRSV序列具有较低的同源性 (<92%) 并被确定为来自1,3,5.1和8.7.7系的重组物.
- FJLIUY-2017诱导了典型的PRRSV临床症状,较高的病毒量,严重的肺病变和20%的死亡率.
- PRRSV2/CN/G8/2018引起了中度临床症状,没有死亡率;两种菌株都没有被高免疫血清对常见疫苗菌株 (JXA1-R和英格尔瓦克PRRS MLV) 中和.
结论:
- 与PRRSV2/CN/G8/2018相比,FJLIUY-2017在小猪中表现出更高的致病性,其特点是持续发烧,病毒血量升高和死亡率.
- 不同PRRSV-2系之间的重组事件可能导致高度致病性PRRSV变种的出现.
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