牛 (Bos taurus) 和野牛 (Bison bison) 是保守的逆转移素重组产物
Gleb Yu Kosovsky1, Galina V Glazko2, Olga I Skobel1
1Department of Biotechnology, Afanas'ev Research Institute of Fur-Bearing Animal Breeding and Rabbit Breeding, Moscow, Russia.
Frontiers in veterinary science
|May 15, 2025
概括
移动遗传元素,特别是三位数逆转移素重组产物,产生微RNA,对农场动物化至关重要. 这些因素影响牛和野牛在经济上重要的特征,有助于了解遗传资源管理.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 管理农场动物遗传资源需要了解与养相关的基因组特征.
- 移动遗传元素,特别是丰富的微RNA,在动物化中发挥着作用.
研究的目的:
- 为了比较养牛和野生野牛之间的重组产物和微RNA.
- 研究移动遗传元素在化过程和特征形成中的作用.
主要方法:
- 在复合产品 (LINE/ERV/LINE序列) 和微RNA的体比较.
- 使用养牛 (Bos taurus) 和野牛 (Bison bison) 的参考基因组.
- 分析保存的基因块和特定物种的微RNA家族.
主要成果:
- 在牛和野牛中都发现了高度保存的重组产物.
- 牛产生了129个microRNAs,野牛51个,共享50个;79个牛特有的microRNAs,主要是mir-30家族.
- Mir-30家族与影响农产品数量和质量的特征有关.
结论:
- 三位数逆转移素重组产物固定在牛和野牛基因组中.
- 这些产品可能有助于在化过程中在人工选择下适应.
- 跨种类的微RNA差异化影响了监管网络和经济上重要的特征.
相关概念视频
Overview of Transposition and Recombination
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
DNA-only Transposons
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
LTR Retrotransposons
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
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Non-LTR Retrotransposons
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Exon Recombination
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...
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Transposons
Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...


