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相关概念视频

The DNA Replication Fork01:02

The DNA Replication Fork

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An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
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Replication in Eukaryotes01:29

Replication in Eukaryotes

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In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
12.8K
Chromosome Replication02:31

Chromosome Replication

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Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
8.6K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.7K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.7K
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

4.6K
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
4.6K
Replication in Prokaryotes01:32

Replication in Prokaryotes

23.9K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
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相关实验视频

Updated: May 17, 2025

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

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额外的复制起源导致细胞周期特定的DNA复制叉速度.

Ole Skovgaard1

  • 1Department of Science and Environment, Roskilde University, Roskilde, Denmark.

Frontiers in microbiology
|May 15, 2025
PubMed
概括

在大肠杆菌中,复制分叉速度 (RFS) 是动态变化的,而不是以前认为的恒定的. 核酸供应限制了RFS,影响了DNA复制动力学.

科学领域:

  • 分子生物学分子生物学
  • 微生物学 微生物学
  • 遗传学 遗传学 是一个

背景情况:

  • 在大肠杆菌中,复制分叉速度 (RFS) 传统上被认为是恒定的.
  • 从 oriC 进行双向复制通常会产生两个复制分叉.
  • 在缓慢生长的条件下,细胞资源受到压力,影响DNA复制.

研究的目的:

  • 为了研究细胞周期内的RFS的动态性质.
  • 挑战埃舍里希亚大肠杆菌中恒定RFS的范式.
  • 探索改变的复制叉号对RFS的影响.

主要方法:

  • 使用了一种大肠杆菌菌株与额外的异胎性oriC (oriX).
  • 分析了来自oriC和oriX的DNA复制启动,创建多达四个分叉.
  • 使用深度测序数据的标记频率分析计算RFS.

主要成果:

  • 随着四个活跃的分叉,RFS减少了大约三分之一,随着一个活跃的分叉增加了四分之一.
  • 在复制周期期间观察到RFS的2倍变化.
  • 延迟启动或增加dNTP池使RFS变化正常化.
关键词:
复制DNA复制DNA复制DNA复制DnaAA DnaA 是一个细胞循环中的细胞循环.在 dNTP 池中使用 dNTP.标志频率分析的频率分析这是一个 oriCC.复制分叉速度的速度分叉.

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Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
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Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

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相关实验视频

Last Updated: May 17, 2025

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

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Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
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Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

149

结论:

  • 在复制周期内,RFS不是恒定的,而是动态变化的.
  • 核酸供应是复制分叉速度的主要限制.
  • 这些发现为研究调节DNA复制动力学的因素提供了基础.