相关实验视频

Updated: May 17, 2025

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

复合微生物剂通过殖民根表面和调节根球土壤微生物群体来控制番茄细菌枯

Shuangxi Zhu¹, Xiaojian Chang², Nana Liu¹

¹Xi'an Key Laboratory of Textile Chemical Engineering Auxiliaries, Key Laboratory of Textile Dyeing Wastewater Treatment Universities of Shaanxi Province, School of Environmental and Chemical Engineering, Engineering Research Center of Biological Resources Development and Pollution Control Universities of Shaanxi Province, Xi'an Polytechnic University, Xi'an, China.

Frontiers in microbiology

|May 15, 2025

概括

一种由Enterobacter hormaechei Rs-5和Bacillus subtilis SL-44组成的复合微生物剂 (EB) 通过减少Ralstonia solanacearum和改善土壤健康,有效地管理番茄细菌枯. EB殖民番茄根,增强植物生长和有益的微生物群落.

科学领域:

农业微生物学农业微生物学
植物病理学植物病理学
土壤科学土壤科学

背景情况:

由Ralstonia solanacearum引起的细菌,严重影响番茄幼苗的生长.
生物控制微生物提供了一种可持续的方法来管理番茄细菌枯.

研究的目的:

为了研究一个复合微生物剂 (EB) 的行为,包括红根球中的Enterobacter hormaechei Rs-5和Bacillus subtilis SL-44.
评估EB对土壤微生物群落结构和番茄植物生长的影响.

主要方法:

盘面对抗和实验评估EB对抗Ralstonia solanacearum和细菌枯的疗效.
绝对定量PCR (AQ-PCR) 和扫描电子显微镜分析EB迁移,殖民和生物膜形成.
高通量测序以确定EB对根球微生物群落的影响.根球微生物群落.

主要成果:

EB有效地殖民番茄根,形成生物膜,并向根排泄物表现出化学反应.
EB显著降低了细菌枯的发生率,从77.78%降至22.22%,并改善了番茄苗的生物质和土壤特性.
EB的应用增加了有益细菌的相对丰富性 (例如,Massilia,Pseudomonas,Bacillus,Enterobacter) 和改善了真菌的多样性.

结论:

EB通过减少根球中的Ralstonia solanacearum直接控制番茄细菌枯.
EB通过向根部迁移,有效殖民并调节土壤微生物社区结构来间接管理疾病.

关键词:

由于细菌的原因,它们会枯.复合微生物代理的复合微生物代理根部排泄物根部排泄物土壤微生物社区番茄幼苗番茄苗番茄苗番茄苗

更多相关视频

Adherence of Bacteria to Plant Surfaces Measured in the Laboratory

Adherence of Bacteria to Plant Surfaces Measured in the Laboratory

Published on: June 19, 2018

Monitoring Bacterial Colonization and Maintenance on Arabidopsis thaliana Roots in a Floating Hydroponic System

Monitoring Bacterial Colonization and Maintenance on Arabidopsis thaliana Roots in a Floating Hydroponic System

Published on: May 28, 2019

相关概念视频

The Roles of Bacteria and Fungi in Plant Nutrition

The Roles of Bacteria and Fungi in Plant Nutrition

Plants have the impressive ability to create their own food through photosynthesis. However, plants often require assistance from organisms in the soil to acquire the nutrients they need to function correctly. Both bacteria and fungi have evolved symbiotic relationships with plants that help the species to thrive in a wide variety of environments.

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...