在骨髓瘤中内皮再造:来自患者样本和体外研究的见解
Mélanie Legrand1,2, Sarah Renault1, Gonzague de Pinieux1,2
1Nantes Université, Inserm UMR 1238 F-44000 Nantes, France.
American journal of cancer research
|May 15, 2025
概括
骨髓瘤瘤含有细胞从内皮转换为介质细胞类型,这一过程被称为内皮转换为介质细胞转换 (EndoMT). 这一发现为复杂的瘤微环境和潜在的治疗点提供了新的见解.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 癌症研究 癌症研究
背景情况:
- 骨髓瘤 (OS) 是具有不良预后的侵袭性原发性骨癌,通常通过当前的化疗和手术治疗不充分.
- 瘤微环境,包括其血管化,在OS异质性和进展中起着至关重要的作用.
- 瘤血管改造可能涉及血管生成和内皮细胞转移到介质细胞转变 (EndoMT).
研究的目的:
- 在骨髓瘤瘤微环境中研究内皮质-介质细胞转换 (EndoMT) 的存在和作用.
- 探索骨髓瘤细胞和脑膜细胞在体外内皮细胞中诱导EndoMT的能力.
主要方法:
- 多重免疫组织化学用于检测患者获得的高等级骨髓瘤样本中的内皮和介质细胞标记物.
- 人类静脉内皮细胞 (HUVECs) 暴露于骨髓瘤细胞系和树皮细胞分泌的因素,以建模瘤微环境.
主要成果:
- 在患者骨髓瘤组织中确定了共表达内皮细胞内皮细胞 (CD31/EMCN) 和介质细胞 (ASMA/FSP1) 标记物的内皮细胞.
- 来自骨髓瘤细胞的分泌因子,而不是肌肉细胞,诱导了HUVEC中EndoMT的一些标志性特征,包括增加迁移和中酶体标记表达.
- 诱导的EndoMT在体外没有抑制内皮管形成.
结论:
- 内皮介质细胞存在于骨髓瘤瘤微环境中.
- 骨髓肉瘤细胞可以通过可溶性因子在内皮细胞中诱导部分内皮细胞到介质细胞的过渡.
- 需要进一步的研究来了解EndoMT在骨髓瘤生物学中的调节和功能后果.
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