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开发悬浮液滴微提取方法,用于光谱测量血清铁的测定

Aruna Jyothi Kora^1,2, K Madhavi¹, N N Meeravali^1,2

¹National Centre for Compositional Characterisation of Materials (NCCCM) Bhabha Atomic Research Centre (BARC) Hyderabad Telangana India.

Analytical science advances

|May 15, 2025

概括

这项研究提出了一种新的,敏感的方法,用于用悬浮滴滴微提取方法在小牛血清样本中量化铁 (Fe3+). 该技术提供了准确且无干扰的结果,使其成为常规分析的可行替代方案.

科学领域:

分析化学分析化学
生物化学生化学
频谱光度测量是一种光谱光度测量.

背景情况:

精确量化铁 (Fe3+) 对于生物和临床诊断至关重要.
在生物样本中测定Fe3+的现有方法往往需要大量,并且可能受到干扰.

研究的目的:

开发一种简单,有选择性和敏感的方法,用于在小体积的生物样本,特别是牛血清中量化Fe3+.
优化悬浮液滴微提取 (SDME) 技术,以提高Fe3+的确定性.

主要方法:

悬浮液滴微提取 (SDME) 使用醇作为提取溶剂.
在酸性介质中将Fe3+与氨基 thiocyanate复合,形成有色复合物.
与Aliquat 336一起形成离子对,以加强将其提取到微滴中.
在甲醇中溶解后,在505nm处检测出提取的复合物的光谱光度测定.

主要成果:

优化的参数包括1.5%的盐酸,0.7%的硫酸氨,0.2%的阿利卡特336和500μL的醇.
实现了高回收率 (96.2%-98.8%) 和较低的相对标准偏差 (1.4%-5.0%).
该方法证明了从20-1000 ng/mL的线性,血清检测极限为2.4 ng/mL.
牛血清样本中的Fe3+度在62.7至1582.5 ng/mL之间,与电热原子吸收光谱学结果一致.

结论:

开发的SDME光谱法为在低体积的生物样本中测定Fe3+提供了灵敏,选择性和准确的方法.
该方法克服了传统技术的局限性,提供了诸如低样本量,成本效益和不受金属和生物干扰等优势.
这种方法作为一种有价值的替代方案,用于血清和其他生物流体中铁的常规光谱分析.

关键词:

在ET-AASAS.颜色测量仪的颜色测量方法复杂化剂是一种复杂化剂.铁是铁,铁是铁,铁是铁.血液中的血清是血清.这是光谱光度测量.悬浮液滴微提取悬浮液滴微提取

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