进步大气固体分析探测器质谱应用:优化临床数据集生成的多方面的方法
Liwen Song1, Jasmine G Reese2, Michael A Platt1
1Department of Chemistry, University of Oxford, Chemistry Research Laboratory, 12 Mansfield Rd, Oxford OX1 3TA, UK. claire.vallance@chem.ox.ac.uk.
The Analyst
|May 15, 2025
概括
大气固体分析探头质谱 (ASAP-MS) 提供了快速的临床代谢物分析. 本研究确定了影响ASAP-MS准确性和可重复性的背景噪音和批量效应等关键因素,并提供了优化建议.
科学领域:
- 分析化学 分析化学
- 临床诊断 临床诊断 临床诊断
- 代谢学 代谢学 代谢学
背景情况:
- 快速质谱仪,如大气固体分析探头质谱仪 (ASAP-MS),对临床代谢物分析有希望.
- ASAP-MS提供快速测量,易于使用和低成本,使其适合临床应用.
- 然而,影响准确性和可重现性的实验因素往往被忽视.
研究的目的:
- 评估实验变量对ASAP-MS测量准确性和可重复性的影响.
- 为在临床环境中优化ASAP-MS协议提供建议.
主要方法:
- 评估包括仪器设置,背景噪音,耗材,用户变化和批量效应在内的因素.
- 使用ASAP-MS分析人类大脑和脑脊液样本.
主要成果:
- 确定了背景噪声,耗材,用户差异和批量影响对ASAP-MS数据的重大影响.
- 由于这些因素,测量准确性和可重复性的变化已被证明.
结论:
- 优化测量和清洁协议对于可靠的ASAP-MS结果至关重要.
- 精心选择耗材和强大的批量效应检测/校正是必不可少的.
- 实施这些建议将提高ASAP-MS在临床环境中的可靠性和可重复性.
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