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Updated: Jul 8, 2026

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使用培养的人类细胞系进行重组拓聚酶2生产

Anh T Q Cong1, Matthew J Schellenberg2

  • 1Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA.

Methods in molecular biology (Clifton, N.J.)
|May 15, 2025
PubMed
概括

我们开发了一种可扩展的方法,用于生产高纯度的重组人体二氧化酶2 (TOP2) α和β蛋白. 该方法使用HEK293F细胞和光蛋白融合进行高效的净化和结构分析.

关键词:
一个纳米人体.再组合蛋白质的表达方式.拓二醇酶2的使用方法转化 转化 转化 转化在YFP-tag中使用YFP标签.mCherry-tag的标签是什么意思

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...