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Updated: Jun 27, 2026

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
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Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Published on: January 30, 2018

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通过使用三色单分子FRET解决GPCR中间状态

Léo Bonhomme1, Ecenaz Bilgen2, Caroline Clerté1

  • 1Centre de Biologie Structurale (CBS), University of Montpellier, CNRS, INSERM, Montpellier 34090, France.

Journal of the American Chemical Society
|May 15, 2025
PubMed
概括

这项研究引入了一种新的三重标记方法, 这一进步使研究人员能够以前所未有的细节观察动态蛋白质构造变化,揭示了对复杂生物过程的新见解.

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Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

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Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET10:59

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...