作为生物样本成像的多响应光探针的AIE活性Cyclen-BODIPYs:设计和全面研究
Denis Y Uvarov1, Nina A Sapoletova2, Sergey E Kushnir2
1N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninsky prosp., 119991, Moscow, Russia(1).
Talanta
|May 15, 2025
概括
新的二甲基 (BODIPY) 染料表现出聚合诱导的发射 (AIE),使"开启"光成为可能. 这些水溶性染料在细胞中选择性地标记 lysosomes,为实时成像应用提供了潜力.
科学领域:
- 有机化学 有机化学
- 材料科学是一种材料科学.
- 生物光子学 生物光子学
背景情况:
- 聚合诱导排放 (AIE) 对于开发先进的有机发光材料至关重要.
- 二甲基 (BODIPY) 具有适用于AIE应用的固有聚合特性.
研究的目的:
- 设计和合成基于BODIPY光体的新型水溶性AIE活性染料.
- 研究合成染料的光物理性质和环境敏感性.
- 评估这些染料在生物成像,特别是溶酶体标记方面的潜力.
主要方法:
- 修改BODIPY光体的阿扎马克罗环单位,以制造AIE活性染料.
- 光物理性质的表征,包括光量子产量和环境灵敏度 (含水量,粘度,pH,温度).
- 使用动态光散射 (DLS) 和扫描电子显微镜 (SEM) 分析聚合物形成.
- 使用TD-DFT进行计算研究,以合理化聚合物中的分子排列.
- 在HeLa细胞中进行了体外共聚焦显微镜研究,以检测细胞吸收和溶酶体标记.
主要成果:
- 成功合成了新型水溶性,AIE活性环-BODIPY衍生物 (5a和8a).
- 染料5a和8a表现出高光量子产量和对环境因素的敏感"打开"反应.
- 在水溶液中观察到纳米级聚合物,这得到了DLS和SEM的证实.
- TD-DFT计算提供了对聚合物的分子排列的见解.
- 染料在HeLa细胞中显示了选择性溶解体标记,没有生物相关金属离子的显著干扰.
结论:
- 设计的cyclen-BODIPY衍生物是有效的AIE活性染料,具有可调节的光物理性质.
- 这些染料在活细胞中显示出对溶酶体向和成像的极佳潜力.
- 这些发现表明,在通过光成像研究 lysosomal 动力学和功能方面具有广泛的适用性.
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