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诱导的线粒体功能障碍和Solanum nigrum叶的氧化损伤 L L L

Yue Teng¹, Yi Xiao², Huibo Sun²

¹School of Environment and Ecology, Jiangsu Key Laboratory of Anaerobic Biotechnology, Jiangnan University, Wuxi, 214122, China; Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou, 215009, China.

Plant physiology and biochemistry : PPB

|May 15, 2025

概括

暴露会损害Solanum nigrum叶中的线粒体,损害能量代谢并增加活性氧物种 (ROS). 这项研究提供了对污染土壤的植物修复策略的见解.

科学领域:

环境科学环境科学
植物生物学植物生物学
生物化学生物化学

背景情况:

(Cd) 在Solanum nigrum L.中积聚,主要在细胞壁和囊泡中.
关于Cd对S. nigrum叶子中线粒体的特定毒性作用的研究有限.

研究的目的:

研究Cd积累对S. nigrum线粒体结构和功能的影响.
为增强S. nigrum在Cd污染土壤植物修复中的作用提供理论基础.

主要方法:

用不同度的化 (CdCl2) 处理S. nigrum.
分析线粒体内的Cd含量.
评估线粒体形态,透压,ATP含量,H+-ATPase活性和活性氧物种 (ROS) 水平 (特别是H2O2).
对抗氧化酶活性 (POD和DHAR) 的评估.

主要成果:

线粒体中的Cd含量随着Cd处理度的增加而增加,达到和.
暴露于Cd引起了线粒体的透失衡和形态变化,导致功能障碍.
在200μMCdCl2.2.下观察到线粒体ATP含量 (高达90.65%) 和H+-ATPase活性 (高达80.65%) 的显著下降.
线粒体H2O2水平随着Cd度的增加而大幅增加 (高达405.81%).
抑制细胞呼吸和升高的ROS加剧了线粒体的氧化损伤.
线粒体过氧化酶 (POD) 和脱酸盐减少酶 (DHAR) 显示出显著的耐受性对Cd压力.

结论:

会导致S. nigrum叶子中线粒体的显著功能障碍和氧化损伤.
这些发现凸显了Cd对细胞能量代谢和植物氧化应激反应的有害影响.
了解这些机制对于优化使用S. nigrum的植物修复策略至关重要.

关键词:

积累的积累的.这是一种重金属,重金属.过度积累装置的工厂线粒体功能障碍线粒体功能障碍氧化应激是一种氧化应激.

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