细颗粒物 (PM2.5) 诱导微RNA-192-5p,导致球损伤
Melina Yarbakht1, George Sarau2, Yanyi Xu3
1Department of Nephrology and Hypertension, Uniklinikum Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Erlangen, Germany.
Ecotoxicology and environmental safety
|May 15, 2025
概括
空气污染,特别是细颗粒物 (PM2.5),可能通过引起炎症和改变microRNA-192-5p的表达,从而影响细胞功能,从而触发膜性淋巴细胞炎 (MGN).
科学领域:
- 腎臟病學 (nephrology) 是一種醫學專業.
- 环境健康 环境健康
- 分子生物学分子生物学
背景情况:
- 流行病学研究表明,空气污染与膜性结膜炎 (MGN) 之间存在联系.
- 确切的因果关系和潜在的病理机制,包括抗脂酶A2受体 (PLA2R1) 抗体和microRNA-192-5p的作用,仍然不清楚.
- 在MGN病原发生过程中,这些分子变化的触发因素是未知的.
研究的目的:
- 调查细颗粒物 (PM2.5) 在膜性淋巴结膜炎 (MGN) 病变发生过程中的潜在作用.
- 描述PM2.5并评估其对球功能和超结构的 in vitro 和 in vivo 影响.
主要方法:
- 使用各种光谱和显微镜技术对PM2.5进行表征.
- 培养的人类受体细胞,斑马鱼和小鼠暴露于PM2.5.5.的暴露.
- 评估淋巴细胞标记物,基因表达 (microRNA-192-5p,NPNT,PLA2R1),功能 (蛋白尿,白蛋白尿) 和淋巴细胞超结构.
- 在斑马鱼中通过BulkRNAseq和qPCR分析炎症反应和氧化应激.
主要成果:
- 暴露于PM2.5降低了细胞特异性标记物和上调的microRNA-192-5p.
- 在PM2.5暴露后,观察到神经红素 (NPNT) 的下调和PLA2R1的上调.
- 暴露于PM2.5的斑马鱼幼虫表现出,蛋白尿,球膜损伤以及炎症和氧化应激标志物的增加.
- 暴露于环境PM2.5的小鼠出现了淋巴细胞损伤,白色素尿和肺微RNA-192-5p上调.
结论:
- 通过炎症途径,PM2.5可能会促进MGN的发展.
- PM2.5可以诱导microRNA-192-5p,影响对球细胞功能至关重要的基因表达.
- 这些发现表明空气污染和MGN病原体之间的潜在因果关系.
相关概念视频
01:19Fineness Modulus
221
The fineness modulus (FM) of aggregate is a numerical index that measures the coarseness or fineness of the particles. It is calculated by adding the cumulative percentages of aggregate retained on each of a specified series of sieves and dividing the sum by 100.
Consider performing sieve analysis on sand through a set of ASTM sieves. The weight of aggregate retained in each sieve and pan placed at the bottom is recorded, as given in Column B of Table 1.
To determine the fineness modulus of...
Consider performing sieve analysis on sand through a set of ASTM sieves. The weight of aggregate retained in each sieve and pan placed at the bottom is recorded, as given in Column B of Table 1.
To determine the fineness modulus of...
221
01:15Fineness of Cement
112
The fineness of cement directly influences the rate of hydration, as the hydration begins at the surface of the cement particles. In addition to hydration, the fineness of cement is vital for various properties of concrete including workability, gypsum requirement, and long-term behavior. The fineness of cement is represented in terms of the specific surface of cement which is typically measured in square meters per kilogram, with several methods available for this determination.
Direct...
Direct...
112
01:16Precipitate Formation and Particle Size Control
625
In precipitation gravimetry, the precipitating agent should react specifically or selectively with the analyte. While a specific reagent reacts with the analyte alone, a selective reagent can react with a limited number of chemical species.
The obtained precipitate should be either a pure substance of known composition or easily converted to one by a simple process, such as ignition or drying. In addition, the precipitate should be insoluble and easily filterable. In general, filterability...
The obtained precipitate should be either a pure substance of known composition or easily converted to one by a simple process, such as ignition or drying. In addition, the precipitate should be insoluble and easily filterable. In general, filterability...
625
00:53Filtration
741
Filtration is a physical separation process that involves passing a suspension through a porous medium to separate solids from fluids. During filtration, solids collect on the porous medium while liquids, also collectively known as the filtrate, pass through. The filtration medium is selected based on the filtration purpose, quantity, and nature of the precipitate. The general criteria for a suitable filtering medium are that it is inert, mechanically strong, nonabsorbent toward dissolved...
741