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一种生物信息学驱动的方法来识别生物标志物并阐明2型糖尿病与肺结核同时发生的病原性

Yan Liu¹, Yonglan Pu², Jie Wang³

¹Clinical Medical Center for Tuberculosis, Beijing Chest Hospital, Capital Medical University, Beijing, China.

Scientific reports

|May 15, 2025

概括

2型糖尿病 (T2DM) 和肺结核 (PTB) 的并发症与不良结果有关. 这项研究确定了五个关键基因 (C1QA,MMP8,MMP9,CD248,LINC00278) 作为PTB+T2DM的潜在诊断标记.

科学领域:

生物医学研究的研究.
基因组学就是基因组学.
传染病和免疫学传染病和免疫学

背景情况:

2型糖尿病 (T2DM) 和肺结核 (PTB) 的同时感染导致更高的治疗失败和死亡率.
了解这种并发症的分子机制对于开发有效的预防和治疗策略至关重要.

研究的目的:

通过生物信息分析识别T2DM (PTB+T2DM) PTB的关键基因,途径和潜在诊断标记.
为了验证在PTB+T2DM患者中发现的基因的表达水平.

主要方法:

进行了权重基因共同表达网络分析 (WGCNA) 和基因本体学/KEGG通路分析.
生物信息分析比较了PTB+T2DM患者和健康对照者之间的免疫细胞透和药物敏感性.
定量实时PCR (qRT-PCR),西部抹杀 (WB) 和ELISA用于体外验证.

主要成果:

五个关键基因 (C1QA,CD248,LINC00278,MMP8,MMP9) 与PTB+T2DM有关.
显著的途径包括细胞外矩阵-受体相互作用和炎症信号传递.
在PTB+T2DM患者的外周血液中,C1QA,MMP8和MMP9被上调,而CD248被下调.

结论:

C1QA,MMP8,MMP9,CD248和LINC00278显示出作为PTB+T2DM的诊断标记物的潜力.
该研究确定了PTB+T2DM的分子连接和潜在的治疗点.
结果为未来的研究和临床应用提供了参考,用于管理这种并发症.

关键词:

C1QQ 一个问题在C1QAQA中,在MMP8中,MMP8是MMP8.在MMP9中,MMP9是MMP9.肺结核是一种肺结核.2 型糖尿病 2 型糖尿病权重基因共同表达网络分析.

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