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基于电化学表面等离子体共振的生物传感器,通过不同的合策略检测α-胎蛋白

Bhawana Thakur¹, Amarnath Chellachamy Anbalagan¹, Pallavi Koyande¹

¹Chemistry Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India.

Scientific reports

|May 15, 2025

概括

这项研究开发了一种用于肝癌检测的电化学表面等离子体共振 (ESPR) 生物传感器. 乙二胺/甲 (EDA/GA) 策略为检测α-fetoprotein (AFP) 生物标志物提供了最高的灵敏度.

科学领域:

生物医学工程生物医学工程
分析化学分析化学
生物传感器技术技术

背景情况:

电化学表面等离子体共振 (ESPR) 生物传感器对于实时,快速的癌症检测至关重要.
阿尔法-胎蛋白 (AFP) 是肝癌的关键生物标志物,需要敏感的检测方法.

研究的目的:

设计和比较三个合策略来固定ESPR生物传感器上的AFP抗体.
为了评估传感器对肝癌生物标志物检测的性能.

主要方法:

开发了一个ESPR生物传感器,使用金涂玻璃传感器盘.
通过三种策略固定了AFP抗体:EDC/NHS,EDA/GA和PANI/GA.
通过电化学阻抗光谱学研究固定效率.

主要成果:

EDA/GA策略产生了最高的灵敏度 (28°/(ng/ml)) 与0.5-3 ng/ml线性范围.
根据EDC/NHS的策略,提供了更广泛的线性范围 (5-70 ng/ml),具有合理的灵敏度 (2.12°/...ng/ml)).
基于EDC/NHS的传感器通过ELISA验证,用于在人类血清样本中检测AFP.

结论:

选择合策略显著影响ESPR生物传感器用于癌症生物标志物检测的性能.
这项研究为选择最佳策略进行AFP临床分析提供了洞察力.
开发的生物传感器显示出准确和高效的肝癌诊断的前景.

关键词:

生物标志物生物标志物生物传感器是一种生物传感器.癌症检测癌症检测电化学阻抗是一种电化学阻抗.表面等离子体共振是什么?

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